Journal of Helminthology

Research Papers

Factors affecting the reliability of the McMaster technique

Alison Dunna1 and Anne Keymera1

a1 Department of Zoology,University of Oxford, Oxford OXI 3PS, England.

Abstract

Factors detraction from the reliability of faecal egg counts based on the McMaster technique include variation in flotation time (interval between loading chamber and counting eggs) and sample dilution (ratio of faecal material to salt solution). We recommend standardization of both these variables as normal laboratory procedure, and propose optima of a 30 minute flotation time and a sample dilution of 15 ml salt solution/ g faeces for use of the McMaster technique in the estimation of the fecundity of Heligmosomoides polygyrus (Nematoda).

(Accepted June 04 1986)