Hostname: page-component-7c8c6479df-fqc5m Total loading time: 0 Render date: 2024-03-28T20:43:24.761Z Has data issue: false hasContentIssue false

An attempt to implement the nematode-trapping fungus Duddingtonia flagrans in biological control of trichostrongyle infections of first year grazing calves

Published online by Cambridge University Press:  05 June 2009

J. Wolstrup
Affiliation:
Department of Ecology and Molecular Biology, Microbiology Section, Royal Veterinary and Agricultural University, 21 Rolighedsvej, DK-1870 Frederiksberg C, Denmark
J. Grønvold
Affiliation:
Department of Ecology and Molecular Biology, Section of Zoology, Royal Veterinary and Agricultural University, 21 Rolighedsvej, DK-1870 Frederiksberg C, Denmark
S.A. Henriksen
Affiliation:
National Veterinary Laboratory, 27 Bülowsvej, DK-1790 Copenhagen V, Denmark
P. Nansen
Affiliation:
Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
M. Larsen
Affiliation:
Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
H.O. Bøgh
Affiliation:
Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark
B. Ilsøe
Affiliation:
Centre for Experimental Parasitology, Department of Veterinary Microbiology, Royal Veterinary and Agricultural University, 13 Bülowsvej, DK-1870 Frederiksberg C, Denmark

Abstract

An attempt was made to control Ostertagia ostertagi by feeding the nematode-trapping fungus Duddingtonia flagrans (DSM 6703) to grazing calves. One group of calves (group E) was fed the fungal material in the first two months of the grazing season while another group was a non-treated control group (group C). Group E showed significantly lower faecal egg count in August and September. On four occasions in July and September, the herbage larval counts were significantly lower on the plot with the fungal-treated group than those recorded on the control plot. The average abomasal larval and adult worm counts were significantly reduced in August in group E and the average total worm count in the abomasum of group E was reduced by 87% in August compared to the non-treated group C. In October, the difference in average abomasal worm counts between group E and C was insignificant. Due to weight loss at the end of the grazing season, the control group showed a significantly lower average weight increase.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 1994

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Grønvold, J., Wolstrup, J., Henriksen, S.A. & Nansen, P. (1987) Field experiments on the ability of Arthrobotrys oligospora (Hyphomycetales) to reduce the number of larvae of Cooperia oncophora (Trichostrongylidae) in cow pats and surrounding grass. Journal of Helminthology 61, 6571.CrossRefGoogle ScholarPubMed
Grønvold, J., Nansen, P., Henriksen, S.A., Thylin, J. & Wolstrup, J. (1988) The capability of the predacious fungus Arthrobotrys oligospora (Hyphomycetales) to reduce numbers of infective larvae of Ostertagia ostertagi (Trichostrongylidae) in cow pats and herbage during the grazing season in Denmark. Journal of Helminthology 62, 271280.CrossRefGoogle ScholarPubMed
Grønvold, J., Henriksen, S.A., Nansen, P., Wolstrup, J. & Thylin, J. (1989) Attempts to control infection with Ostertagia ostertagi (Trichostrongylidae) in grazing calves by adding mycelium of the nematode-trapping fungus Arthrobotrys oligospora (Hyphomycetales) to cow pats. Journal of Helminthology 63, 115126.CrossRefGoogle Scholar
Grønvold, J., Wolstrup, J., Nansen, P., Henriksen, S.A., Larsen, M. & Bresciani, J. (1993a) Biological control of nematode parasites in cattle with nematode-trapping fungi: a survey of Danish studies. Veterinary Parasitology 48, 311325.CrossRefGoogle ScholarPubMed
Grønvold, J., Wolstrup, J., Larsen, M., Henriksen, S.A. & Nansen, P. (1993b) Biological control of Ostertagia ostertagi by feeding selected nematode-trapping fungi to calves. Journal of Helminthology 67, 3136.CrossRefGoogle ScholarPubMed
Henriksen, S.A. & Aagaard, K. (1976) En enkel flotations- og McMastermetode. Nordisk Veterinærmedicin 28, 392397.Google Scholar
Jørgensen, R.J. (1975) Isolation of infective Dictyocaulus larvae from herbage. Veterinary Parasitology 1, 6167.CrossRefGoogle Scholar
Larsen, M., Wolstrup, J., Henriksen, S.A., Dackman, C., Grønvold, J. & Nansen, P. (1991) In vitro stress selection of nematophagous fungi for biocontrol of parasitic nematodes in ruminants. Journal of Helminthology 65, 193200.CrossRefGoogle ScholarPubMed
Larsen, M., Wolstrup, J., Henriksen, S.A., Grønvold, J. & Nansen, P. (1992) In vivo passage through calves of nematophagous fungi selected for biocontrol of parasitic nematodes. Journal of Helminthology 66, 137141.CrossRefGoogle ScholarPubMed
Mwegoha, W.M. & Jørgensen, R.J. (1977) Recovery of infective 3rd stage larvae of Haemonchus contortus and Ostertagia ostertagi by migration in agar gel. Acta Veterinaria Scandinavica 18, 293299.CrossRefGoogle ScholarPubMed
Ross, J.G., Purcell, D.A., Dow, C. & Todd, J.R. (1967) Experimental infections of calves with Trichostrongylus axei; the course and development of infection and lesions in low level infections. Research in Veterinary Science 8, 201206.CrossRefGoogle ScholarPubMed
Satrija, F. & Nansen, P. (1993) Experimental concurrent infections with Ostertagia ostertagi and Cooperia oncophora in the calf. Research in Veterinary Science 55, 9297.CrossRefGoogle ScholarPubMed
Siegel, S. (1956) Non-parametric statistics for the behavioural sciences. 312 pp. Tokyo, McGraw-Hill Kogakusha Ltd.Google Scholar