a1 Departmento de Parasitologia, Facultad de Farmacia, Universidad Complutense, 28040-Madrid, Spain
a2 Centro Nacional de Biotecnología, cl Serrano 115-bis, 28006-Madrid, Spain
a3 Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, GU24 0NF, England
Comparison of antibody response and antigen recognition was made by ELISA and western-blot analysis in pig experimental infections by T-l and T-3 Spanish Trichinella isolates. Two groups of Iberian pigs were experimentally infected with 150 larvae/kg body weight of GM-1 and C-76 Spanish Trichinella isolates as representatives of T-1 and T-3 gene pools respectively. Antibody levels and antigen recognition were measured on days –14, 0, 6, 16, 20, 27, 34, 49, 63 and 82 after infection by ELISA and western-blotting assays. Antibody response against C-76 infection was significantly delayed and lower than against GM-1. The two Trichinella isolates were indistinguishable, however, by western blotting analysis, although recognition of larval antigens was quantitatively higher than adult ones. Interestingly, the principle larval antigenic components recognized by pigs were those recognized by the monoclonal antisera NIM-M1. Finally, there were no serological patterns indicative of the stage of infection (“antibody windows”) discriminating, for example between early versus late infections.
(Accepted February 12 1992)