Hostname: page-component-8448b6f56d-mp689 Total loading time: 0 Render date: 2024-04-17T19:51:08.491Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparative study of assays detecting circulating immune complexes and specific antibodies in patients infected with Toxocara canis

Published online by Cambridge University Press:  05 June 2009

C. Aguila ,
C. Cuéllar ,
S. Fenoy  and
J. L. Guillén
C. Aguila
Affiliation:
Departamento de Biologia Molecular, Inst. Llorente-Fides, 28035 Madrid, Spain
C. Cuéllar
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, U.C.M., 28040 Madrid, Spain
S. Fenoy
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, U.C.M., 28040 Madrid, Spain
J. L. Guillén
Affiliation:
Departamento de Parasitología, Facultad de Farmacia, U.C.M., 28040 Madrid, Spain
Get access Rights & Permissions [Opens in a new window]

Abstract

A sandwich ELISA method using previously described E/S antigen-specific monoclonal antibodies has been developed to detect circulating immune complexes in patients infected with Toxocara canis. This technique could be used for the study of the dynamics of the parasite-host relationship, as we believe the detection of immune complexes and/or soluble antigen to be an improvement over detection of antibodies only. In this parasitosis, antibodies may be present in residual levels for prolonged periods after active infection.

Keywords

ELISAToxocara caniscirculating immune complexesmanimmunodiagnosis.
Type
Research Papers
Information
Journal of Helminthology , Volume 61 , Issue 3 , September 1987 , pp. 196 - 202
Copyright
Copyright © Cambridge University Press 1987

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abdel-Hameed, A. A. (1984) Effect of thiabendazole on the migration of Toxocara canis larvae in the mouse. Journal of Parasitology, 70, 226–231.CrossRefGoogle ScholarPubMed
Aguila, C., Cuellar, C. & Guillen, J. L. (1986) Hybridomas specific for Toxocara canis excretory-secretory antigen: Immunological and biochemical properties. Sixth International Congress of Parasitology. Brisbane, Australia. Abstract 177.Google Scholar
Glickman, L. T., Schantz, P., Dombroske, R. & Cypess, R. (1978) Evaluation of serodiagnostic tests for visceral larva migrans. American Journal of Tropical Medicine and Hygiene, 27, 492–498.CrossRefGoogle ScholarPubMed
Glickman, L. T., Cypess, R., Hiles, D. & Gressner, T. (1979) Toxocara-specific antibody in the serum and aqueous humor of a patient with presumed ocular and visceral toxocariasis. American Journal of Tropical Medicine and Hygiene, 28, 29–35.CrossRefGoogle ScholarPubMed
Glickman, L. T., Schantz, P. M. & Grieve, R. B. (1986) In: “Immunodiagnosis of Parasitic Disease” Vol. 16 (Editors, Walls, K. W. and Schantz, P. M.) 1986, 201–225. Academic Press. London.Google Scholar
Guillen, J. L., Cuellar, C. & Aguila, C. (1986) Larva Migratoria Visceral: estudio mediante ELISA de tres grupos familiares. Pediatrika, 6, 28–32.Google Scholar
Maizels, R. M., Savigny, D. & Ogilvie, B. M. (1984) Characterization of surface and excretory-secretory antigens of Toxocara canis infective larvae. Parasite Immunology, 6, 23–27.CrossRefGoogle ScholarPubMed
Matsumura, K. & Endo, R. (1982) Enzyme-Linked Immunosorbent Assay for toxocariasis, its application to the sera of children. Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene, 225, 402–406.Google Scholar
Matsumura, K. & Endo, R. (1983) Seroepidemiological study on toxocaral infection in man by Enzyme-Linked Immunosorbent Assay. Journal of Hygiene, 90, 61–65.CrossRefGoogle ScholarPubMed
Matsumura, K., Kazuta, Y., Endo, R. & Tanaka, K. (1984) Detection of circulating toxocaral antigens in dogs by sandwich enzyme immunoassay. Immunology, 51, 609–613.Google ScholarPubMed
Olson, L. J. (1960) Serology of visceral larva migrans: “in vitro” larval precipitate test. Texas Reports on Biology and Medicine, 18, 473–479.Google ScholarPubMed
Richards, B., Olson, L. J. & Box, Q. T. (1962) Survey of Galveston children for antibodies to Toxocara canis. Journal of Parasitology, 48, 501.CrossRefGoogle Scholar
Savigny, D. H. (1975) In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara E/S antigen for use in serodiagnostic tests for VLM. Journal of Parasitology, 61, 781–782.CrossRefGoogle Scholar
Speiser, F. & Gottstein, B. (1984) A collaborative study on larval E/S antigens of Toxocara canis for the immunodiagnosis of human toxocariasis with ELISA. Acta Tropica, 41, 361–372.Google Scholar
Tonz, M., Speiser, F. & Tonz, O. (1983) Toxocariasis in Swiss children. Schweizerische Medizinische wochenschrift, 113, 1500–1507.Google ScholarPubMed
Van Knapen, F. V., Van Leusden, J. V., Polderman, A. M. & Franchimont, J. H. (1983) Visceral larva migrans: examinations by means of ELISA of human sera for antibodies to excretory-secretory antigens of the L-2 Toxocara canis. Zeitschrift für Parasitenkunde, 69, 113–118.CrossRefGoogle Scholar
Woodruff, A. W. & Savigny, D. H. (1982) Toxocaral and toxoplasmal antibodies in cat breeders and in Icelanders exposed to cats but not to dogs. British Medical Journal, 284, 309–310.CrossRefGoogle ScholarPubMed