British Journal of Nutrition

Full Papers

Effect of folic acid on prenatal alcohol-induced modification of brain proteome in mice

Yajun Xua1 c1, Yunan Tanga1 and Yong Lia1

a1 Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100083, China

Abstract

Maternal alcohol consumption during pregnancy can induce central nervous system abnormalities in the fetus, and folic acid supplementation can reverse some of the effects. The objective of the present study was to investigate prenatal alcohol exposure-induced fetal brain proteome alteration and the protective effect of folic acid using proteomic techniques. Alcohol (5·0 g/kg) was given intragastrically from gestational day (GD) 6 to15, with or without 60·0 mg folic acid/kg given intragastrically during GD1–16 to pregnant Balb/c mice. The control group received distilled water only. Results of litter evaluation on GD18 showed that supplementation of folic acid reversed the prevalence of microcephaly induced by alcohol. Proteomic analysis indicated that, under the dosage of the present investigation, folic acid mainly reversed the alcohol-altered proteins involved in energy production, signal pathways and protein translation, which are all important for central nervous system development.

(Received December 19 2006)

(Revised July 05 2007)

(Accepted July 05 2007)

Correspondence:

c1 Corresponding author: Dr Yajun Xu, fax +86 10 82801575, email yajun_xucn@126.com

Footnotes

Abbreviations: CNS, central nervous system; FA, folic acid; FAS, fetal alcohol syndrome; GD, gestational day; TCTP, translationally controlled tumour protein