Parasitology



A phylogeny based on three mitochondrial genes supports the division of Schistosoma intercalatum into two separate species


R. A. KANE a1c1, V. R. SOUTHGATE a1, D. ROLLINSON a1, D. T. J. LITTLEWOOD a2, A. E. LOCKYER a2, J. R. PAGÈS a3, L. A. TCHUEM TCHUENTÉ a4 and J. JOURDANE a3
a1 Biomedical Parasitology Division, Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
a2 Parasitic Worms Division, Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
a3 Centre de Biologie et d'Ecologie Tropicale et Méditerranéenne (UMR 5555 du CNRS), Université de Perpignan, Av. De Villeneuve, 66860 Perpignan Cedex, France
a4 Laboratoire de Biologie Générale, Faculté des Sciences, Université de Yaoundé I, B.P. 812 Yaoundé, Cameroun

Article author query
kane r   [PubMed][Google Scholar] 
southgate v   [PubMed][Google Scholar] 
rollinson d   [PubMed][Google Scholar] 
littlewood d   [PubMed][Google Scholar] 
lockyer a   [PubMed][Google Scholar] 
pages j   [PubMed][Google Scholar] 
tchuem tchuente l   [PubMed][Google Scholar] 
jourdane j   [PubMed][Google Scholar] 

Abstract

Two recognized strains of Schistosoma intercalatum, one from the Democratic Republic of Congo (DRC), formerly Zaire, and the other from Cameroon, have been investigated using DNA sequences from 3 mitochondrial genes, cytochrome oxidase subunit 1 (cox1), NADH dehydrogenase subunit 6 (nad6) and the small ribosomal RNA gene (rrnS). In addition, partial DNA sequences from the nuclear large subunit ribosomal RNA gene (lsrDNA) were included within the study. Although partial lsrDNA alone reveals little taxonomic information, phylogenetic analysis of the mitochondrial data demonstrates a clear dichotomy between the 2 purported strains and it is proposed that they should be treated as distinct taxa. The ‘original’ S. intercalatum now falls relatively basal in the S. haematobium group, while the proposed new species is more derived and sister taxon to S. bovis and S. curassoni.

(Received January 30 2003)
(Revised February 14 2003)
(Accepted February 14 2003)


Key Words: Schistosoma intercalatum; molecular diversity; phylogeny; cox1; nad6; rrnS; lsrDNA.

Correspondence:
c1 Biomedical Parasitology Division, Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD. Tel: +44 20 7942 5152. Fax: +44 20 7942 5347. E-mail: rak@nhm.ac.uk


Metrics