CJO - Abstract - Host oyster tissue extracts modulate <em>in vitro</em> protease expression and cellular differentiation in the protozoan parasite, <em>Perkinsus marinus</em>

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    Host oyster tissue extracts modulate in vitro protease expression and cellular differentiation in the protozoan parasite, Perkinsus marinus
    E. A. MACINTYRE, C. G. EARNHART and S. L. KAATTARI (2003).
    Parasitology, Volume 126, Issue 04, April 2003 pp 293-302 http://journals.cambridge.org/action/displayAbstract?aid=148183

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Parasitology (2003), 126 : 293-302 Cambridge University Press
Copyright © 2003 Cambridge University Press
doi:10.1017/S003118200200286X (About doi)
Published online by Cambridge University Press 30 Apr 2003
 
Parasitology (2003), 126:293-302 Cambridge University Press
Copyright © 2003 Cambridge University Press
DOI 10.1017/S003118200200286X

Host oyster tissue extracts modulate in vitro protease expression and cellular differentiation in the protozoan parasite, Perkinsus marinus

E. A. MACINTYRE a1, C. G. EARNHART a1 and S. L. KAATTARI a1c1
a1 Department of Environmental and Aquatic Animal Health, School of Marine Science, Virginia Institute of Marine Science, College of William and Mary, Gloucester Point, VA 23062–1346, USA

Medline query on article authors

macintyre e
earnhart c
kaattari s

Abstract

Perkinsus marinus is responsible for a chronic disease (Dermo) of the Eastern oyster, Crassostrea virginica. In order to simulate the in vivo environment more closely, a chemically defined medium (JL-ODRP-3) was supplemented with tissue homogenate extracts or plasma from oysters possessing varying degrees of susceptibility to P. marinus infection. In media supplemented with extracts from highly susceptible oysters (C. virginica), P. marinus cells secreted elevated amounts of a set of low molecular weight serine proteases (LMP: 30–45 kDa) as assessed by enhanced digestion within gelatin-substrate SDS–PAGE gels. Oyster species of low susceptibility (C. gigas and C. ariakensis) did not exhibit this ability to upregulate P. marinus LMP expression. Oyster extract supplementation also led to pronounced changes in P. marinus cellular morphology, such that the cells were comparable to those observed within naturally infected oysters.

(Received May 25 2002)
(Revised October 23 2002)
(Accepted October 29 2002)


Key Words: Perkinsus marinus; protease; Crassostrea sp.; differentiation; in vitro culture.

Correspondence:
c1 Tel: +804 684 7362. Fax: +804 684 7186. E-mail: kaattari@vims.edu

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