Zygote

Article

Laser blastocyst biopsy for preimplantation diagnosis in the human

A. Veigaa1 c11, M. Sandalinasa11, M. Benkhalifaa12, M. Boadaa11, M. Carreraa13, J. Santalóa14, P.N. Barria11 and Y. Ménézoa11

a1 Institut Universitari Dexeus and UAB, Barcelona, Spain and Labaoratoire Marcel Mérieux, Lyon, France

Summary

A new methodology for blastocyst biopsy that uses a 1.48μm in diode laser is described. Trophectoderm cells are biopsied after laster zona drilling and culture, fixed and processed for fluorescent in situ hybridisation (FISH) analysis. Preliminary results on the efficiency of the procedure and blastocyst recovery rate are promising. Blastocyst laser biopsy is a useful tool in preimplantation genetic diagnosis (PGD) as it allows a more reliable diagnosis and widens the diagnostic possibilities on account of the higher number of cells obtained in the biopsy.

(Received June 10 1997)

(Accepted July 15 1997)

Correspondence:

c1 A. Veiga, Reproductive Medicine Service, Department of Obstetrics and Gynaecology, Institut Universitari Dexeus, Barcelona, Spain.

Footnotes

1 Reproductive Medicine Service, Department of Obstetrics and Gynaecology, Institut Universitari Dexeus, Barcelona, Spain.

2 Genetics and IVF, Laboratoire Marcel Mérieux, Lyon, France.

3 Centre de Patologia Celular, Institut Universitari Dexeus, Barcelona, Spain.

4 Dept Biologia Cellulari Fisiologia UAB, Barcelona, Spain.

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