International Journal of Tropical Insect Science

Research Paper

The butterfly Danaus chrysippus (Lepidoptera: Nymphalidae) in Kenya is variably infected with respect to genotype and body size by a maternally transmitted male-killing endosymbiont (Spiroplasma)

Jeremy K. Herrena1 c1, Ian Gordona2, Peter W. H. Hollanda1 and David Smitha3

a1 Department of Zoology, University of Oxford, South Parks Road, Oxford OX1 3PS, UK

a2 ICIPE—African Insect Science for Food and Health, PO Box 30772-00100, Nairobi, Kenya

a3 Natural History Museum, Eton College, Windsor SL4 6EW, UK

Abstract

Female-biased sex ratios in Danaus chrysippus (Linnaeus) (family Nymphalidae, subfamily Danainae, tribe Danaini) populations are attributed to the action of an endosymbiotic ‘male-killer’ bacterium of the genus Spiroplasma. In stark contrast to the extensive geographic range of their host, the Spiroplasma appears to be restricted to East Africa, where four African D. chrysippus subspecies exist sympatrically and form a hybrid zone. In this study, specimens collected at three sample sites within the hybrid zone were screened for Spiroplasma infection. The findings demonstrate that, within the hybrid zone, the frequency of Spiroplasma infection varies both spatially and temporally. Host genotype at three biallelic wing pattern loci, representative of subspecific genetic divergence, is correlated to Spiroplasma infection. Linkage between the frequency of the recessive a allele and Spiroplasma is established, suggesting the presence of a linked allele controlling infection susceptibility. In addition, a negative correlation between D. chrysippus forewing length and Spiroplasma infection is identified, suggesting that infection has a deleterious effect on body size.

(Accepted May 21 2007)

Correspondence

c1 E-mail: jeremy.herren@jesus.oxon.org

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