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The structure and formation of metacercarial cysts in the trematode family Microphallidae Travassos 1920

Published online by Cambridge University Press:  05 June 2009

K.V. Galaktionov ,
I.I. Malkova ,
S.W.B. Irwin ,
D.H. Saville  and
J.G. Maguire
K.V. Galaktionov
Affiliation:
Murmansk Marine Biological Institute, Russian Academy of Sciences Kola Scientific Centre, 17 Vladimirskaya Street, 183101, Murmansk, Russia:
I.I. Malkova
Affiliation:
Murmansk Marine Biological Institute, Russian Academy of Sciences Kola Scientific Centre, 17 Vladimirskaya Street, 183101, Murmansk, Russia:
S.W.B. Irwin*
Affiliation:
School of Applied Biological and Chemical Sciences, University of Ulster at Jordanstown, Shore Road, Newtownabbey, County Antrim, Northern Ireland, BT37 OQB
D.H. Saville
Affiliation:
School of Applied Biological and Chemical Sciences, University of Ulster at Jordanstown, Shore Road, Newtownabbey, County Antrim, Northern Ireland, BT37 OQB
J.G. Maguire
Affiliation:
School of Applied Biological and Chemical Sciences, University of Ulster at Jordanstown, Shore Road, Newtownabbey, County Antrim, Northern Ireland, BT37 OQB
*
*Author for correspondence.
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Abstract

This study deals with the formation of the metacercarial cysts of four microphallid trematodes, Maritrema subdolum, M. arenaria, Levinseniella brachysoma and Microphallus claviformis. The first observable cyst was present around Maritrema arenaria 18 h p.i. (post-infection). The other species had not developed a cyst by day 8 p.i. but their cysts were apparent by day 16 p.i. These were bi-layered and that of M. subdolum was thicker than those of L. brachysoma and Microphallus claviformis of the same age. The structure of older cysts varied substantially between the four species. Microphallus claviformis and Maritrema subdolum cysts were fully formed at 30 days p.i. Like those of M. arenaria they were bi-layered, the outer layer (up to 3 μm thick) being electron-dense and the inner one (up to 7 μm thick) being less electron-dense. The cysts of fully formed L. brachysoma metacercariae were much more complex, composed of four layers, one of which was divisible into three sub-layers. It was concluded that the outer cyst layer was the product of secretory granules which were previously identified in cercarial tegument. The inner, thicker layer was derived from several sources. These included small tegument vesicles produced over the entire surface of the metacercariae, larger fragments of tegument released from the anterio-ventral region and material liberated from the metacercarial excretory bladder. This heterogeneous material accumulated in the cyst lumen for some time before becoming polymerized to form the thick inner layer or layers of the metacercarial cysts.

Type
Research Papers
Information
Journal of Helminthology , Volume 71 , Issue 1 , March 1997 , pp. 13 - 20
Copyright
Copyright © Cambridge University Press 1997

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References

Abbas, M.K. & Cain, G.D. (1987) Actin and intermediatesized filaments of the spines and cytoskeleton of Schistosoma mansoni. Parasitology Research 73, 66–74.Google Scholar
Alderson, G.D. (1975) Ultrastructure and histochemistry of the metacercarial cysts of Spelotrema nicolli (Microphallidae: Trematoda). International Journal for Parasitology 5, 659–665.Google Scholar
Asanji, M.F. & Williams, M.O. (1973) The structure and histochemistry of some trematode metacercarial cysts. Journal of Helminthology 47, 353–368.Google Scholar
Benjamin, L.R. & James, B.L. (1987) The development of the metacercariae of Maritrema linguilla Jag. 1908 (Digenea; Microphallidae) in the intermediate host Ligia oceanica (L). Parasitology 94, 221–231.Google Scholar
Bock, D. (1988) Formation, histochemistry and ultrastructure of the metacercarial cyst wall of Plagiorchis species 1 (Trematoda, Plagiorchiidae). International Journal for Parasitology 18, 379–388.Google Scholar
Cable, R.M. (1974) Phylogeny and taxonomy of trematodes with reference to marine species. pp. 173–193in Vernberg, W.B. (Ed.) Symbioses in the sea. The Belle W. Buruch Library in Marine Science, No. 2. Columbia, South Carolina, University of South Carolina Press.Google Scholar
Cohen, C., Reinhardt, B., Castellani, L., Norton, P. & Stirewalt, M. (1982) Schistosome surface spines are ‘crystals’ of actin. Journal of Cell Biology 95, 987–988.Google Scholar
Galaktionov, K.V. & Dobrovolskii, A.A. (1987) Hermaphroditic generation of Trematodes. Leningrad, Nauka Press (in Russian).Google Scholar
Galaktionov, K.V. & Krasnodembskii, E.G. (1990) Glandulous formations of microphallid trematode cercariae. Vestnik Leningradskogo Universiteta, Ser. 3 2, 3–10 (in Russian).Google Scholar
Galaktionov, K.V. & Malkova, I.I. (1994) The glands of trematode cercariae of the family Microphallidae Travassos, 1920. International Journal for Parasitology 24, 595–604.Google Scholar
Galaktionov, K.V. & Malkova, I.I. (1995) Changes in the excretory bladder ultrastructure during the morphogenesis of Levinseniella brachysoma (Trematoda: Microphallidae) metacercariae. Journal of Helminthology 69, 203–212.Google Scholar
Galaktionov, K.V., Malkova, I.I., Irwin, S.W.B., Saville, D.H. & Maguire, J.G. (1996) Developmental changes in the tegument of four microphallid metacercariae in their second (crustacean) intermediate hosts. Journal of Helminthology 70, 201–210.Google Scholar
Irwin, S.W.B. (1983) In vitro excystment of the metacercaria of Maritrema arenaria (Digenea: Microphallidae). International Journal for Parasitology 13, 191–196.Google Scholar
Leong, C.H.D. & Howell, M.J. (1970) Formation and structure of the cyst wall of Stictodora lari (Trematoda: Heterophyidae). Zeitschrift für Parasitenkunde 35, 340–350.Google Scholar
Mitchell, C.W. (1974) Ultrastructure of the metacercarial cyst of Posthodiplostomum minimum (MacCallum, 1921). Journal of Parasitology 60, 67–74.Google Scholar
Popiel, I. (1976) The ultrastructure of the metacercaria of Cercaria stunkardi Palombi, 1934 (Digenea: Opecoelidae) in an experimental intermediate host, Amphithoe rubricata. Norwegian Journal of Zoology 24, 353–364.Google Scholar
Ramasamy, P. & Paniker, S.L. (1991) Studies on the chemical nature of the cyst wall of Microphallus madrasensis. Journal of Helminthology 65, 111–119.Google Scholar
Saville, D.H. & Irwin, S.W.B. (1991) A light and electron microscope study on in vitro excystation of Microphallus abortivus (Digenea: Microphallidae) metacercariae. Parasitology Research 77, 82–85.Google Scholar
Smyth, J.D. & Halton, D.W. (1983) The physiology of trematodes, 2nd edn.Cambridge University Press.Google Scholar
Stanier, J.E., Woodhouse, M.A. & Griffin, R.L. (1968) Light and electron-microscopic observations on the metacercaria of a Spelotrema sp. (Trematoda: Microphallidae) encysted in Carcinus maenas. Journal of Invertebrate Pathology 10, 269–282.Google Scholar
Stein, P.C. & Lumsden, R.D. (1971a) The ultrastructure of developing metacercarial cysts of Ascocotyle leighi Burton, 1956 (Heterophyidae). Proceedings of the Helminthological Society of Washington 38, 1–10.Google Scholar
Stein, P.C. & Lumsden, R.D. (1971b) An ultrastructure and cytochemical study of metacercarial cyst development in Ascocotyle pachycystis Schoeder and Leigh, 1965. Journal of Parasitology 57, 1231–1246.Google Scholar
Strong, P. & Cable, R.M. (1972) Fine structure and development of the metacercarial cyst in Microphallus opacus. Journal of Parasitology 58, 92–98.Google Scholar