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Effects of a novel anti-exospore monoclonal antibody on microsporidial Nosema bombycis germination and reproduction in vitro

Published online by Cambridge University Press:  19 June 2007

F. ZHANG ,
X. LU ,
V. S. KUMAR ,
H. ZHU ,
H. CHEN ,
Z. CHEN  and
J. HONG
F. ZHANG
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
X. LU*
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
V. S. KUMAR
Affiliation:
P.G. Department of Studies and Research in Sericulture, Karnatak University, Dharwad-580 003, India
H. ZHU
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
H. CHEN
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
Z. CHEN
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
J. HONG
Affiliation:
Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China
*
*Corresponding author: Laboratory of Invertebrate Pathology, Zhejiang University, Hangzhou 310029, China. Fax: +86 571 86971697. E-mail: xmlu@zju.edu.cn
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Summary

A monoclonal antibody (mAb) 3C2, against an exospore protein of the microsporidium Nosema bombycis (N. bombycis) was prepared, and its effects on microsporidial germination and reproduction in vitro were studied. MAb 3C2 was effective in inhibiting the germination and subsequent infection of Bombyx mori cells compared to the control mAb. The antigen was isolated by 2-dimensional gel electrophoresis. Immunoblotting revealed it to be an 84 kDa protein corresponding to pI (7·2) on the 2-D gel. The present results suggest that the antibodies can be used for diagnostic purposes and for developing new therapeutic strategies in controlling microsporidian diseases.

Keywords

microsporidiaNosema bombycismonoclonal antibody2D-PAGEgerminationreproductionin vitro
Type
Research Article
Information
Parasitology , Volume 134 , Issue 11 , October 2007 , pp. 1551 - 1558
Copyright
Copyright © Cambridge University Press 2007

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