Chinese Journal of Agricultural Biotechnology

Research Article

Analysis of isozymes related to energy metabolism of adult Tegillarca granosa

Su Xiu-Ronga1 c1, Lv Zhen-Minga1, Li Tai-Wua1, Liu Zhi-Minga2 and Paul K. Chiena3

a1 Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China

a2 Department of Biology, Eastern New Mexico University, NM 88130, USA

a3 Department of Biology, San Francisco University, CA 23430, USA

Abstract

The isozymes of 10 enzymes connected with energy metabolism in Tegillarca granosa were analysed by vertical polyacrylamide gel electrophoresis. Esterase and α-amylase are enzymes related to energy intake, their activities were high in the digestive gland. Malate dehydrogenase, malic enzyme, isocitrate dehydrogenase, succinate dehydrogenase, alcohol dehydrogenase, lactate dehydrogenase, 6-phosphogluconate dehydrogenase (G-6-PDH) and adenosine triphosphatase (ATPase) are enzymes related to energy metabolism. The main energy supply of T. granosa comes from aerobic respiration; anaerobic metabolism and the pentose phosphate pathway take an auxiliary role in energy metabolism. The high activity of G-6-PDH in T. granosa might mean a considerable proportion of carbohydrates metabolized through this pathway. This reaction could provide abundant NADP for metabolism in T. granosa. Compared with other shellfish, T. granosa had lower activity of ATPase, which might have some relationship with the amnicolous life and low motility of this animal.

(Received May 08 2006)

(Accepted July 09 2006)

Correspondence:

c1 Corresponding author. E-mail: suxiurong@nbu.edu.cn

Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(2): 242–246