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Genetic relationships among violet root rot fungi as revealed by hyphal anastomosis and sequencing of the rDNA ITS regions

Published online by Cambridge University Press:  09 May 2002

Yukari UETAKE ,
Masao ARAKAWA ,
Hitoshi NAKAMURA ,
Tomoya AKAHIRA ,
Akira SAYAMA ,
Lian-Heng CHEAH ,
Ikuko OKABE  and
Naoyuki MATSUMOTO
Yukari UETAKE
Affiliation:
Bio-oriented Technology Research Advancement Institution, National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Japan. Current addresses: Bio-oriented Technology Research Advancement Institution, National Grassland Research Institute, Nishinasuno 329-2793, Japan.
Masao ARAKAWA
Affiliation:
Bio-oriented Technology Research Advancement Institution, National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Japan. Faculty of Agriculture, Meijo University, Shiogamaguchi, Nagoya 468-0073, Japan.
Hitoshi NAKAMURA
Affiliation:
Bio-oriented Technology Research Advancement Institution, National Institute for Agro-Environmental Sciences, Tsukuba 305-8604, Japan.
Tomoya AKAHIRA
Affiliation:
Aomori Apple Experiment Station, Kuroishi 036-0332, Japan.
Akira SAYAMA
Affiliation:
Akita Agricultural Experiment Station, Yuuwa 010-1231, Japan.
Lian-Heng CHEAH
Affiliation:
Crop & Food Research, PB11 600, Palmerston North, New Zealand.
Ikuko OKABE
Affiliation:
National Institute for Agro-Environmental Sciences, 3-1-3 Kan-non dai, Tsukuba 305-8604, Japan.
Naoyuki MATSUMOTO
Affiliation:
National Institute for Agro-Environmental Sciences, 3-1-3 Kan-non dai, Tsukuba 305-8604, Japan.
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Abstract

Genetic relationships among the violet root rot fungi were studied by hyphal anastomosis and sequencing of the ITS-5·8S rDNA regions. Twelve isolates of Helicobasidium mompa, one isolate each of H. purpreum and H. compactum, two isolates of Helicobasidium sp., eight isolates of Rhizoctonia crocorum, and three isolates of R. violacea anastomosed in most combinations, resulting in the death of fused cells and adjacent cells. Phylogenetic analyses of the ITS-5·8S rDNA regions separated 31 violet root rot fungal isolates into five lineages: H. mompa from Japan and Korea; R. crocorum from Europe and New Zealand; H. purpureum, R. crocorum, and R. violacea from Europe; Helicobasidium sp. from Japan; and H. compactum from the USA. Within each lineage there was no more than one nucleotide substitution, and similarity ranged from 81·8% to 98·2% between lineages. The discrepancy between the results from the two methods are discussed in terms of the biological species concept in the complex.

Type
Research Article
Information
Mycological Research , Volume 106 , Issue 2 , February 2002 , pp. 156 - 163
Copyright
© The British Mycological Society 2002

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