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Foveolar Müller Cells of the Pied Flycatcher: Morphology and Distribution of Intermediate Filaments Regarding Cell Transparency

Published online by Cambridge University Press:  01 March 2016

Lidia Zueva ,
Tatiana Golubeva ,
Elena Korneeva ,
Vladimir Makarov ,
Igor Khmelinskii Open the ORCID record for Igor Khmelinskii [Opens in a new window]  and
Mikhail Inyushin
Lidia Zueva
Affiliation:
Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 194223 St-Petersburg, Russia
Tatiana Golubeva
Affiliation:
Department of Vertebrate Zoology, Lomonosov Moscow State University, 119992 Moscow, Russia Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485 Mosсow, Russia
Elena Korneeva
Affiliation:
Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485 Mosсow, Russia
Vladimir Makarov
Affiliation:
Department of Physics, University of Puerto Rico, San Juan, PR 00931, USA
Igor Khmelinskii
Affiliation:
Faculty of Sciences and Technology, University of the Algarve, Faro 8005-139, Algarve, Portugal
Mikhail Inyushin*
Affiliation:
Department of Physiology, Central University of the Caribbean, Bayamon, PR 00956, USA
*
*Corresponding author. mikhail.inyushin@uccaribe.edu
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Abstract

Specialized intermediate filaments (IFs) have critical importance for the clearness and uncommon transparency of vertebrate lens fiber cells, although the physical mechanisms involved are poorly understood. Recently, an unusual low-scattering light transport was also described in retinal Müller cells. Exploring the function of IFs in Müller cells, we have studied the morphology and distribution pattern of IFs and other cytoskeletal filaments inside the Müller cell main processes in the foveolar part of the avian (pied flycatcher) retina. We found that some IFs surrounded by globular nanoparticles (that we suggest are crystallines) are present in almost every part of the Müller cells that span the retina, including the microvilli. Unlike IFs implicated in the mechanical architecture of the cell, these IFs are not connected to any specific cellular membranes. Instead, they are organized into bundles, passing inside the cell from the endfeet to the photoreceptor, following the geometry of the processes, and repeatedly circumventing numerous obstacles. We believe that the presently reported data effectively confirm that the model of nanooptical channels built of the IFs may provide a viable explanation of Müller cell transparency.

Keywords

IFsavian retinaMüller cellstransparency
Type
Biological Applications
Information
Microscopy and Microanalysis , Volume 22 , Issue 2 , April 2016 , pp. 379 - 386
Copyright
© Microscopy Society of America 2016 

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