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Live Cell Imaging of Actin Dynamics in the Filamentous Fungus Aspergillus nidulans

Published online by Cambridge University Press:  16 February 2016

Zachary Schultzhaus ,
Laura Quintanilla ,
Angelyn Hilton  and
Brian D. Shaw
Zachary Schultzhaus
Affiliation:
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, USA
Laura Quintanilla
Affiliation:
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, USA
Angelyn Hilton
Affiliation:
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, USA
Brian D. Shaw*
Affiliation:
Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77843, USA
*
*Corresponding author. bdshaw@tamu.edu
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Abstract

Hyphal cells of filamentous fungi grow at their tips in a method analogous to pollen tube and root hair elongation. This process, generally referred to as tip growth, requires precise regulation of the actin cytoskeleton, and characterizing the various actin structures in these cell types is currently an active area of research. Here, the actin marker Lifeact was used to document actin dynamics in the filamentous fungus Aspergillus nidulans. Contractile double rings were observed at septa, and annular clusters of puncta were seen subtending growing hyphal tips, corresponding to the well-characterized subapical endocytic collar. However, Lifeact also revealed two additional structures. One, an apical array, was dynamic on the face opposite the tip, while a subapical web was dynamic on the apical face and was located several microns behind the growth site. Each was observed turning into the other over time, implying that they could represent different localizations of the same structure, although hyphae with a subapical web grew faster than those exhibiting an apical array. The subapical web has not been documented in any filamentous fungus to date, and is separate from the networks of F-actin seen in other tip-growing organisms surrounding septa or stationary along the plasmalemma.

Keywords

lifeactsubapical actin webcontractile actin ringseptumlive cell imaging
Type
Special Issue on Imaging Plant Biology
Information
Microscopy and Microanalysis , Volume 22 , Supplement 2 , April 2016 , pp. 264 - 274
Copyright
© Microscopy Society of America 2016 

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Footnotes

a

These authors contributed equally to this manuscript.

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