Epidemiology and Infection



Multilocus sequence typing of Neisseria meningitidis directly from cerebrospinal fluid


P.  KRIZ  a1 c1, J.  KALMUSOVA  a1 and J.  FELSBERG  a2
a1 National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Prague, Czech Republic
a2 Institute of Microbiology, Czech Academy of Science, Prague, Czech Republic

Abstract

MLST typing of Neisseria meningitidis directly from clinical material was introduced in the National Reference Laboratory for Meningococcal Infections in Prague. Four cerebrospinal fluid samples were obtained from patients with suspected meningococcal invasive disease. In all samples, all classical laboratory methods gave negative results and the only positive method was PCR, which revealed Neisseria meningitidis group C (two specimens) and group B (two specimens), respectively. MLST performed directly from cerebrospinal fluid revealed that the strains causing the two group C infections were of sequence type (ST) 11, while the two group B infections were characterized as ST-32 and ST-33 respectively. Multi-locus sequence typing of meningococci directly from clinical material offers the opportunity to improve further the surveillance of meningococcal disease and has now been introduced into the routine portfolio of tests employed at the national reference laboratory of the Czech Republic.

(Accepted November 22 2001)


Correspondence:
c1 Author for correspondence: National Reference Laboratory for Meningococcal Infections, National Institute of Public Health, Srobarova 48, 100 42 Prague 10, Czech Republic.


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