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Hydrogen Peroxide Vapor Decontamination in a Patient Room Using Feline Calicivirus and Murine Norovirus as Surrogate Markers for Human Norovirus

Published online by Cambridge University Press:  10 February 2016

Torsten Holmdahl*
Affiliation:
Infectious Diseases Unit, Department of Clinical Sciences, Lund University, Skåne University Hospital SUS, Malmö, Sweden
Mats Walder
Affiliation:
Medical Microbiology, Department of Laboratory Medicine, Lund University, Skåne University Hospital SUS, Malmö, Sweden
Nathalie Uzcátegui
Affiliation:
Scandinavian Micro Biodevices, Farum, Denmark
Inga Odenholt
Affiliation:
Infectious Diseases Unit, Department of Clinical Sciences, Lund University, Skåne University Hospital SUS, Malmö, Sweden
Peter Lanbeck
Affiliation:
Infectious Diseases Unit, Department of Clinical Sciences, Lund University, Skåne University Hospital SUS, Malmö, Sweden
Patrik Medstrand
Affiliation:
Department of Translational Medicine, Lund University, Malmö, Sweden
Anders Widell
Affiliation:
Department of Translational Medicine, Lund University, Malmö, Sweden
*
Address correspondence to Torsten Holmdahl, MD, Department of Infectious Diseases, Skåne University Hospital SUS, 20502 Malmö, Sweden (Torsten.holmdahl@skane.se).

Abstract

OBJECTIVE

To determine whether hydrogen peroxide vapor (HPV) could be used to decontaminate caliciviruses from surfaces in a patient room.

DESIGN

Feline calicivirus (FCV) and murine norovirus (MNV) were used as surrogate viability markers to mimic the noncultivable human norovirus. Cell culture supernatants of FCV and MNV were dried in triplicate 35-mm wells of 6-well plastic plates. These plates were placed in various positions in a nonoccupied patient room that was subsequently exposed to HPV. Control plates were positioned in a similar room but were never exposed to HPV.

METHODS

Virucidal activity was measured in cell culture by reduction in 50% tissue culture infective dose titer for FCV and by both 50% tissue culture infective dose titer and plaque reduction for MNV.

RESULTS

Neither viable FCV nor viable MNV could be detected in the test room after HPV treatment. At least 3.65 log reduction for FCV and at least 3.67 log reduction for MNV were found by 50% tissue culture infective dose. With plaque assay, measurable reduction for MNV was at least 2.85 log units.

CONCLUSIONS

The successful inactivation of both surrogate viruses indicates that HPV could be a useful tool for surface decontamination of a patient room contaminated by norovirus. Hence nosocomial spread to subsequent patients can be avoided.

Infect Control Hosp Epidemiol 2016;37:561–566

Type
Original Articles
Copyright
© 2016 by The Society for Healthcare Epidemiology of America. All rights reserved 

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