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Repair of dentin defects from DSPP knockout mice by PILP mineralization

Published online by Cambridge University Press:  26 January 2016

Hamid Nurrohman ,
Kunkio Saeki ,
Karina M.M. Carneiro ,
Yung-Ching Chien ,
Sabra Djomehri ,
Sunita P. Ho ,
Chunlin Qin ,
Laurie B. Gower ,
Sally J. Marshall  and
Grayson W. Marshall
...Show all authors
Hamid Nurrohman
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA
Kunkio Saeki
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA
Karina M.M. Carneiro
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA
Yung-Ching Chien
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA
Sabra Djomehri
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA
Sunita P. Ho
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA
Chunlin Qin
Affiliation:
Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis, Texas A&M University Baylor College of Dentistry, Dallas, Texas 75246, USA
Laurie B. Gower
Affiliation:
Department of Materials Science & Engineering, University of Florida, Gainesville, Florida 32611, USA
Sally J. Marshall
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA
Grayson W. Marshall
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA
Stefan Habelitz*
Affiliation:
Department of Preventive and Restorative Dental Sciences, University of California, San Francisco, San Francisco, California 94143, USA
*
a)Address all correspondence to this author. e-mail: Stefan.Habelitz@ucsf.edu
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Abstract

Dentinogenesis imperfecta type II (DGI-II) lacks intrafibrillar mineral with severe compromise of dentin mechanical properties. A Dspp knockout (Dspp–/–) mouse, with a phenotype similar to that of human DGI-II, was used to determine if poly-L-aspartic acid [poly(ASP)] in the “polymer-induced liquid-precursor” (PILP) system can restore its mechanical properties. Dentin from six-week old Dspp–/– and wild-type mice was treated with CaP solution containing poly(ASP) for up to 14 days. Elastic modulus and hardness before and after treatment were correlated with mineralization from Micro x-ray computed tomography (Micro-XCT). Transmission electron microscopy (TEM)/Selected area electron diffraction (SAED) were used to compare matrix mineralization and crystallography. Mechanical properties of the Dspp–/– dentin were significantly less than wild-type dentin and recovered significantly (P < 0.05) after PILP-treatment, reaching values comparable to wild-type dentin. Micro-XCT showed mineral recovery similar to wild-type dentin after PILP-treatment. TEM/SAED showed repair of patchy mineralization and complete mineralization of defective dentin. This approach may lead to new strategies for hard tissue repair.

Keywords

dentinogenesis imperfectamineralizationrepair and engineeringmechanical properties and nanoindentation
Type
Biomineralization and Biomimetics Articles
Information
Journal of Materials Research , Volume 31 , Issue 3 , 15 February 2016 , pp. 321 - 327
Copyright
Copyright © Materials Research Society 2016 

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Footnotes

Contributing Editor: Michelle L. Oyen

References

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