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Deficiencies in extrusion of the second polar body due to high calcium concentrations during in vitro fertilization in inbred C3H/He mice

Published online by Cambridge University Press:  27 October 2015

Yuki Ohta ,
Yoshikazu Nagao ,
Naojiro Minami ,
Satoshi Tsukamoto  and
Seiji Kito
Yuki Ohta
Affiliation:
Science Service Inc., 4-9-1 Anagawa Inage-ku, Chiba, 263–8555, Japan. Department of Technical Support and Development, National Institute of Radiological Sciences, 4-9-1 Anagawa Inage-ku, Chiba, 263–8555, Japan. University Farm, Faculty of Agriculture, Utsunomiya University, Shimokomoriya 443, Mohka, Tochigi 321–4415, Japan. Present address: Yakult Central Institute, 5–11 Izumi, Kunitachi-shi, Tokyo, 186–8650, Japan.
Yoshikazu Nagao
Affiliation:
University Farm, Faculty of Agriculture, Utsunomiya University, Shimokomoriya 443, Mohka, Tochigi 321–4415, Japan.
Naojiro Minami
Affiliation:
Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606–8502, Japan.
Satoshi Tsukamoto
Affiliation:
Department of Technical Support and Development, National Institute of Radiological Sciences, 4-9-1 Anagawa Inage-ku, Chiba, 263–8555, Japan.
Seiji Kito*
Affiliation:
Department of Technical Support and Development, National Institute of Radiological Sciences, 4–9-1 Anagawa Inage-ku, Chiba, 263–8555, Japan. Department of Technical Support and Development, National Institute of Radiological Sciences, 4-9-1 Anagawa Inage-ku, Chiba, 263–8555, Japan.
*
All correspondence to: S. Kito. Department of Technical Support and Development, National Institute of Radiological Sciences, 4–9-1 Anagawa Inage-ku, Chiba, 263–8555, Japan. Tel: +81 43 206 3059. Fax: +81 43 206 4093. E-mail: sk126@nirs.go.jp
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Summary

Successful in vitro fertilization (IVF) of all inbred strains of laboratory mice has not yet been accomplished. We have previously shown that a high calcium concentration improved IVF in various inbred mice. However, we also found that in cumulus-free ova of C3H/He mice such IVF conditions significantly increased the deficiency of extrusion of the second polar body (PBII) in a dose-dependent manner (2% at 1.71 mM and 29% at 6.84 mM, P < 0.05) and that PBII extrusion was affected by high calcium levels at 2–3 h post-insemination. While developmental competence of ova without PBII extrusion to blastocysts after 96 h culture was not affected, a significant reduction in the nuclear number of the inner cell mass was observed in blastocyst fertilized under high calcium condition. We also examined how high calcium concentration during IVF affects PBII extrusion in C3H/He mice. Cumulus cells cultured under high calcium conditions showed a significantly alleviated deficient PBII extrusion. This phenomenon is likely to be specific to C3H/He ova because deficient PBII extrusion in reciprocal fertilization between C3H and BDF1 gametes was observed only in C3H/He ova. Sperm factor(s) was still involved in deficient PBII extrusion due to high calcium concentrations, as this phenomenon was not observed in ova activated by ethanol. The cytoskeletal organization of ova without PBII extrusion showed disturbed spindle rotation, incomplete formation of contractile ring and disturbed localization of actin, suggesting that high calcium levels affect the anchoring machinery of the meiotic spindle. These results indicate that in C3H/He mice high calcium levels induce abnormal fertilization, i.e. deficient PBII extrusion by affecting the cytoskeletal organization, resulting in disturbed cytokinesis during the second meiotic division. Thus, use of high calcium media for IVF should be avoided for this strain.

Keywords

C3H/He miceCalcium and cytoskeletal organizationIn vitro fertilizationInbredOvaSecond polar body
Type
Research Article
Information
Zygote , Volume 24 , Issue 4 , August 2016 , pp. 603 - 616
Copyright
Copyright © Cambridge University Press 2015 

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