Epidemiology and Infection

Original Papers

MRSA

The epidemiology and molecular characterization of methicillin-resistant staphylococci sampled from a healthy Jordanian population

A. G. AL-BAKRIa1 c1, H. AL-HADITHIa1, V. KASABRIa2, G. OTHMANa1, A. KRIEGESKORTEa3 and K. BECKERa3

a1 Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, The University of Jordan, Amman-Jordan

a2 Department of Biopharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, The University of Jordan, Amman-Jordan

a3 Institute of Medical Microbiology, University Hospital Münster, Münster, Germany

SUMMARY

The prevalence of natural carriage and molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS) isolates in a Jordanian community were investigated. The MRSA nasal carriage rate in 227 healthy volunteers was 7·5% and the majority (81%) of MRSA harboured the resistance element SCCmec type IVe and were of a novel spa type t9519 (76%); other significant spa gene types were t223 (14·7%) and t044 (5·9%). All MRSA isolates were susceptible to other classes of antibiotics, and tested positive for at least three virulence factor encoding genes, but only two harboured the pvl gene. MR-CoNS carriage was 54·2% and these isolates were characterized by single, double and untypable SCCmec elements, with Staphylococcus epidermidis SCCmec type IVa predominating. Of eight subjects with nasal co-colonization of MR-CoNS + MRSA, three shared SCCmec type IV in both groups of organisms. This is the first report of methicillin-resistant staphylococci carriage in a Jordanian community and its findings are important for epidemiological study and infection control measures of these organisms.

(Received September 09 2012)

(Revised December 29 2012)

(Accepted December 31 2012)

(Online publication January 23 2013)

Key words

  • Jordan;
  • MR-CoNS;
  • MRSA;
  • SCCmec ;
  • spa

Correspondence

c1 Author for correspondence: Dr A. G. Al-Bakri, Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, The University of Jordan, Amman-Jordan. (Email: agbakri@ju.edu.jo)

Metrics