Plant Genetic Resources

Research Article

Phenotypic evaluation of the Chinese mini-mini core collection of peanut (Arachis hypogaea L.) and assessment for resistance to bacterial wilt disease caused by Ralstonia solanacearum

Huifang Jianga1, Xiaoping Rena1, Yuning Chena1, Li Huanga1, Xiaojing Zhoua1, Jiaquan Huanga1, Lutz Froenickea2, Jiujiang Yua3, Baozhu Guoa4 c1 and Boshou Liaoa1 c1

a1 Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, Hubei 430062, China

a2 UC Davis Genome Center, University of California, Davis, CA 95616, USA

a3 Southern Regional Research Center, USDA-Agricultural Research Service, New Orleans, LA 70124, USA

a4 Crop Protection and Management Research Unit, USDA-Agricultural Research Service, Tifton, GA 31793, USA

Abstract

In order to utilize germplasm resources more efficiently for peanut (Arachis hypogaea L.) genetic improvement, a core collection of 576 accessions and a primary mini core collection of 298 accessions were developed previously from a collection of 6839 cultivated peanut lines stored at the Oil Crops Research Institute of Chinese Academy of Agricultural Sciences at Wuhan. For an efficient evaluation and characterization of the most useful agronomic and disease-resistant traits, an even smaller collection of peanut accessions that represent a spectrum of phenotypes could be more desirable. For this reason, a mini-mini core collection with 99 accessions from the core accessions was developed based on the analysis of 21 morphological traits. It was demonstrated that there were no significant differences between the core and mini-mini core collections in 20 out of the 21 morphological traits studied. Further, the mini-mini core collection captured the ranges of all of the 21 traits displayed in the core collection. The newly developed mini-mini core collection was assessed for resistance to bacterial wilt disease caused by Ralstonia solanacearum. Two accessions showing a high level of resistance to bacterial wilt were identified, demonstrating the usefulness of the mini-mini core collection. The mini-mini-core collection provides a more efficient means of germplasm evaluation and will be resequenced as part of the International Peanut Genome Consortium sequencing project at the UC-Davis Genome Center.

(Received August 14 2012)

(Accepted October 30 2012)

(Online publication November 29 2012)

Key Words:

  • Arachis hypogaea; bacterial wilt;
  • mini core collection;
  • Ralstonia solanacearum

Correspondence

c1 Corresponding authors: E-mails: baozhu.guo@ars.usda.gov; lboshou@hotmail.com

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