Hostname: page-component-8448b6f56d-c47g7 Total loading time: 0 Render date: 2024-04-24T10:58:59.560Z Has data issue: false hasContentIssue false
  • English
  • Français

Starch metabolism mutants in barley: A TILLING approach

Published online by Cambridge University Press:  16 March 2011

Riccardo Bovina ,
Valentina Talamè ,
Salvi Silvio ,
Maria Corinna Sanguineti ,
Paolo Trost ,
Francesca Sparla  and
Roberto Tuberosa
Riccardo Bovina
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
Valentina Talamè
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
Salvi Silvio
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
Maria Corinna Sanguineti
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
Paolo Trost
Affiliation:
Department of Experimental Evolutionary Biology, University of Bologna, Bologna, Italy
Francesca Sparla
Affiliation:
Department of Experimental Evolutionary Biology, University of Bologna, Bologna, Italy
Roberto Tuberosa*
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
*
*Corresponding author. E-mail: roberto.tuberosa@unibo.it
Get access Rights & Permissions [Opens in a new window]

Abstract

In this study, the targetting-induced local lesions in genomes approach was used to identify mutants for genes related to starch metabolism in barley. Starch is the major reserve of plants and serves as primary carbohydrate component in human and livestock diets and has also numerous industrial applications. Mutants for biosynthetic or regulatory genes of starch metabolism often produce starch granules with abnormal morphological and molecular features that could be of interest for technological applications. We report the identification of 29 mutations in five starch-related barley genes (Bmy1, GBSSI, LDA1, SSI and SSII) through the molecular screening of TILLMore, a sodium azide-mutagenized population. Almost all the mutations detected were CG–TA transitions and several (c. 60%) implied a change in amino-acid sequence and therefore possible phenotypic effects. Four mutants showed non-sense or splice-junction alterations, which could drastically affect the protein function.

Keywords

Hordeum vulgarereverse-geneticsstarchTILLING
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 170 - 173
Copyright
Copyright © NIAB 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Asano, T, Kunieda, N, Omura, Y, Ibe, H, Kawasaki, T, Takano, M, Sato, M, Furuhashi, H, Mujin, T, Takaiwa, F, Wu, C, Tada, Y, Satozawa, T, Sakamoto, M and Shimada, H (2002) Rice SPK, a calmodulin-like domain protein kinase, is required for storage product accumulation during seed development. The Plant Cell 14: 619–628.CrossRefGoogle ScholarPubMed
Colbert, T, Till, BJ, Tompa, R, Reynolds, S, Steine, MN, Yeung, AT, McCallum, CM, Comai, L and Henikoff, S (2001) High-throughput screening for induced point mutations. Plant Physiology 126: 480–484.CrossRefGoogle ScholarPubMed
Fujita, N, Yoshida, M, Asakura, N, Ohdan, T, Miyao, A, Hirochika, H and Nakamura, Y (2006) Function and characterization of starch synthase I using mutants in rice. Plant Physiology 140: 1070–1084.CrossRefGoogle ScholarPubMed
Greene, EA, Codomo, CA, Taylor, NE, Henikoff, JG, Till, BJ, Reynolds, SH, Enns, LC, Burtner, C, Johnson, JE, Odden, AR, Comai, L and Henikoff, S (2003) Spectrum of chemically induced mutations from a large-scale reverse-genetic screen in Arabidopsis. Genetics 164: 731–740.CrossRefGoogle ScholarPubMed
Hizukuri, S (1996) Starch: analytical aspects. In: Eliasson, AC (ed.) Carbohydrates in Food. New York: Dekker, pp. 347–429.Google Scholar
James, MG, Denyerz, K and Myers, AM (2003) Starch synthesis in the cereal endosperm. Current Opinion in Plant Biology 6: 215–222.CrossRefGoogle ScholarPubMed
Jobling, S (2004) Improving starch for food and industrial applications. Current Opinion in Plant Biology 7: 210–218.CrossRefGoogle ScholarPubMed
Lemke, H, Burghammer, M, Flot, D, Rössle, M and Riekel, C (2004) Structural processes during starch granules hydration by synchrotron radiation microdiffraction. Biomacromolecules 5: 1316–1324.CrossRefGoogle ScholarPubMed
McCallum, CM, Comai, L, Greene, EA and Henikoff, S (2000) Targeting Induced Local Lesion IN Genomes (TILLING) for plant functional genomics. Plant Physiology 123: 439–442.CrossRefGoogle ScholarPubMed
Michalska, J, Zauber, H, Buchanan, BB, Cejudo, FJ and Geigenberger, P (2009) NTRC links built-in thioredoxin to light and sucrose in regulating starch synthesis in chloroplasts and amyloplasts. Proceedings of the National Academy of Sciences USA 106: 9908–9913.CrossRefGoogle ScholarPubMed
Ng, PC and Henikoff, S (2003) SIFT: Predicting amino acid changes that affect protein function. Nucleic Acids Research 31: 3812–3814.CrossRefGoogle ScholarPubMed
Olsen, O, Wang, X and von Wettstein, D (1993) Sodium azide mutagenesis: preferential generation of AT/GC transition in the barley Ant18 gene. Proceedings of the National Academy of Sciences USA 90: 8043–8047.CrossRefGoogle Scholar
Sehnke, PC, Chung, HJ, Wu, K and Ferl, RJ (2001) Regulation of starch accumulation by granule-associated plant 14-3-3 proteins. Proceedings of the National Academy of Sciences USA 98: 765–770.CrossRefGoogle ScholarPubMed
Sestili, F, Botticella, E, Bedo, Z, Phillips, A and Lafiandra, D (2009) Production of novel allelic variation for genes involved in starch biosynthesis through mutagenesis. Molecular Breeding 25: 145–154.CrossRefGoogle Scholar
Slade, AJ, Fuerstenberg, SI, Loeffler, D, Steine, MN and Facciotti, D (2005) A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING. Nature Biotechnology 23: 75–81.CrossRefGoogle ScholarPubMed
Talamè, V, Bovina, R, Sanguineti, MC, Tuberosa, R, Lundqvist, U and Salvi, S (2008) TILLMore, a resource for the discovery of chemically induced mutants in barley. Plant Biotechnology Journal 6: 477–485.CrossRefGoogle ScholarPubMed
Taylor, NE and Greene, EA (2003) PARSESNP: A tool for the analysis of nucleotide polymorphisms. Nucleic Acids Research 31: 3808–3811.CrossRefGoogle ScholarPubMed
Till, BJ, Reynolds, SH, Greene, EA, Codomo, CA, Enns, LC, Johnson, JE, Burtner, C, Odden, AR, Young, K, Taylor, NE, Henikoff, JG, Comai, L and Henikoff, S (2003) Large-scale discovery of induced point mutations with high-throughput TILLING. Genome Research 13: 524–530.CrossRefGoogle ScholarPubMed
Umemoto, T, Yano, M, Satoh, H, Shomura, A and Nakamura, Y (2002) Mapping of a gene responsible for the difference in amylopectin structure between japonica-type and indica-type rice varieties. Theoretical and Applied Genetics 104: 1–8.CrossRefGoogle ScholarPubMed
Valerio, C, Costa, A, Marri, L, Issakidis-Bourguet, E, Pupillo, P, Trost, P and Sparla, F (2011) Thioredoxin-regulated {beta}-amylase (BAM1) triggers diurnal starch degradation in guard cells, and in mesophyll cells under osmotic stress. Journal Experimental Botany 62: 545–555.CrossRefGoogle ScholarPubMed
Zeeman, SC, Kossmann, J and Smith, AM (2010) Starch: its metabolism, evolution, and biotechnological modification in plants. Annual Review of Plant Biology 61: 209–234.CrossRefGoogle ScholarPubMed