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Seed longevity in oilseed rape (Brassica napus L.) – genetic variation and QTL mapping

Published online by Cambridge University Press:  15 March 2011

Manuela Nagel ,
Maria Rosenhauer ,
Evelin Willner ,
Rod J. Snowdon ,
Wolfgang Friedt  and
Andreas Börner
Manuela Nagel
Affiliation:
Leibniz Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Gatersleben, Germany
Maria Rosenhauer
Affiliation:
Leibniz Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Gatersleben, Germany
Evelin Willner
Affiliation:
Leibniz Institute for Plant Genetics and Crop Plant Research, Satellite Collections North, Inselstraße 9, Malchow/Poel, Germany
Rod J. Snowdon
Affiliation:
Department of Plant Breeding, Justus Liebig University, Heinrich-Buff-Ring 26–32, Giessen, Germany
Wolfgang Friedt
Affiliation:
Department of Plant Breeding, Justus Liebig University, Heinrich-Buff-Ring 26–32, Giessen, Germany
Andreas Börner*
Affiliation:
Leibniz Institute for Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, Gatersleben, Germany
*
*Corresponding author. E-mail: boerner@ipk-gatersleben.de
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Abstract

Although oilseed rape has become one of the most important oil crops in Europe, little is known regarding the viability of its seed under conditions of long-term storage. We report here an examination of oilseed rape seed longevity performed on a set of 42 accessions housed at the German ex situ genebank at IPK, Gatersleben. A comparison of germination between the accessions stored for 26 years showed that viability was in part genetically determined, since it ranged between 42 and 98%. An attempt was made to define the genetic basis of viability by subjecting a mapping population of doubled haploids to three artificial ageing treatments. Quantitative trait loci (QTL) were detected on six chromosomes: N6, N7, N8, N15, N16 and N18. The chromosomal locations of these QTL were compared with their syntenic regions in Arabidopsis thaliana in order to explore what genes might underlie genetic variation for longevity.

Keywords

Brassica napus Lex situ genebankquantitative trait lociseed longevitysynteny
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 260 - 263
Copyright
Copyright © NIAB 2011

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References

Badani, AG, Snowdon, RJ, Baetzel, R, Lipsa, FD, Wittkop, B, Horn, R, De Haro, A, Font, R, Lühs, W and Friedt, W (2006) Co-localisation of a partially dominant gene for yellow seed colour with a major QTL influencing acid detergent fibre (ADF) content in different crosses of oilseed rape (Brassica napus). Genome 49: 1499–1509.CrossRefGoogle Scholar
Bentsink, L, Alonso-Blanco, C, Vreugdenhil, D, Tesnier, K, Groot, SPC and Koornneef, M (2000) Genetic analysis of seed-soluble oligosaccharides in relation to seed storability of Arabidopsis. Plant Physiology 124: 1595–1604.CrossRefGoogle ScholarPubMed
Clerkx, EJM, Blankestijn-De Vries, H, Ruys, GJ, Groot, SPC and Koornneef, M (2004 a) Genetic differences in seed longevity of various Arabidopsis mutants. Physiologia Plantarum 121: 448–461.CrossRefGoogle Scholar
Clerkx, EJM, El-Lithy, ME, Vierling, E, Ruys, GJ, Blankestijin-De Vries, H, Groot, SPC, Vreugdenhil, D and Koornneef, M (2004 b) Analysis of natural allelic variation of Arabidopsis seed germination and seed longevity traits between the accessions Landsberg erecta and Shakdara, using a new recombinant inbred line population. Plant Physiology 135: 432–443.CrossRefGoogle ScholarPubMed
Debeaujon, I, Leon-Kloosterziel, KM and Koornneef, M (2000) Influence of the testa on seed dormancy, germination, and longevity in Arabidopsis. Plant Physiology 122: 403–413.CrossRefGoogle ScholarPubMed
Delouche, JC and Baskin, CC (1973) Accelerated aging techniques for predicting the relative storability of seed lots. Seed Science and Technology 1: 427–452.Google Scholar
Freitas, RA, Dias, DCFS, Oliveira, MGA, Dias, LAS and Jose, IC (2006) Physiological and biochemical changes in naturally and artificially aged cotton seeds. Seed Science and Technology 34: 253–264.CrossRefGoogle Scholar
Friedt, W and Snowdon, RJ (2010) Oilseed rape. In: Vollmann, J and Rajan, J (eds) Oil crops. Handbook of Plant Breeding, vol. 4. NY: Springer-Verlag, pp. 91–126.Google Scholar
Hampton, JG and TeKrony, DM (eds) (1995) Handbook of Vigour Test Methods. Zürich: International Seed Testing Association.Google Scholar
Hay, FR, Adams, J, Manger, K and Probert, R (2008) The use of non-saturated lithium chloride solutions for experimental control of seed water content. Seed Science and Technology 36: 737–746.CrossRefGoogle Scholar
McDonald, MB (1999) Seed deterioration: physiology, repair and assessment. Seed Science and Technology 27: 177–237.Google Scholar
Nagel, M and Börner, A (2010) The longevity of crop seeds stored under ambient conditions. Seed Science Research 20: 1–12.CrossRefGoogle Scholar
Nagel, M, Vogel, H, Landjeva, S, Buck-Sorlin, G, Lohwasser, U, Scholz, U and Börner, A (2009) Seed conservation in ex-situ genebanks – genetic studies on longevity in barley. Euphytica 170: 1–10.CrossRefGoogle Scholar
Nagel, M, Abdur Rehman Arif, M, Rosenhauer, M and Börner, A (2010) Longevity of seeds – intraspecific differences in the Gatersleben genebank collections. Tagungsband der 60. Tagung der Vereinigung der Pflanzenzüchter und Saatgutkaufleute Österreichs. Raumberg-Gumpenstein (Austria), pp. 179–181.Google Scholar
Nelson, JC (1997) QGene: software for marker-based genomic analysis and breeding. Molecular Breeding 3: 239–245.CrossRefGoogle Scholar
Parkin, IAP, Gulden, SM, Sharpe, AG, Lukens, L, Trick, M, Osborn, TC and Lydiate, DJ (2005) Segmental structure of the Brassica napus genome based on comparative analysis with Arabidopsis thaliana. Genetics 171: 765–781.CrossRefGoogle ScholarPubMed
Priestley, DA and Leopold, AC (1979) Absence of lipid oxidation during accelerated aging of soybean seeds. Plant Physiology 63: 726–729.CrossRefGoogle ScholarPubMed
Rajjou, L, Lovigny, Y, Groot, SPC, Belghaz, M, Job, C and Job, D (2008) Proteome-wide characterization of seed aging in Arabidopsis: a comparison between artificial and natural aging protocols. Plant Physiology 148: 620–641.CrossRefGoogle ScholarPubMed
Tesnier, K, Strookman-Donkers, HM, Van Pijlen, JG, Van der Geest, AHM, Bino, RJ and Groot, SPC (2002) A controlled deterioration test for Arabidopsis thaliana reveals genetic variation in seed quality. Seed Science and Technology 30: 149–165.Google Scholar
Thorlby, G, Veale, E, Butcher, K and Warren, G (1999) Map positions of SFR genes in relation to other freezing-related genes of Arabidopsis thaliana. Plant Journal 17: 445–452.CrossRefGoogle ScholarPubMed
Walters, C, Wheeler, LM and Grotenhuis, JM (2005 a) Longevity of seeds stored in a genebank: species characteristics. Seed Science Research 15: 1–20.CrossRefGoogle Scholar
Walters, C, Hill, LM and Wheeler, LJ (2005 b) Dying while dry: kinetics and mechanisms of deterioration in desiccated organisms. Integrative and Comparative Biology 45: 751–758.CrossRefGoogle ScholarPubMed