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Genetic diversity among Italian melon inodorus (Cucumis melo L.) germplasm revealed by ISSR analysis and agronomic traits

Published online by Cambridge University Press:  15 March 2011

S. Sestili ,
A. Giardini  and
N. Ficcadenti
S. Sestili*
Affiliation:
Agricultural Research Council, Horticultural Crop Research Unit, Via Salaria 1, 63030Monsampolo del Tronto (AP), Italy
A. Giardini
Affiliation:
Agricultural Research Council, Horticultural Crop Research Unit, Via Salaria 1, 63030Monsampolo del Tronto (AP), Italy
N. Ficcadenti
Affiliation:
Agricultural Research Council, Horticultural Crop Research Unit, Via Salaria 1, 63030Monsampolo del Tronto (AP), Italy
*
*Corresponding author. E-mail: saras17@libero.it
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Abstract

The genetic relationships among 13 melon inodorus populations that were collected in southern Italy were assessed using 100 inter-simple-sequence repeat (ISSR) primers and 15 morphological traits. The dihaploid line Nad-1 and the cultivar Charentais-T, both of which belong to the botanical variety cantalupensis, were used as reference accessions in the molecular analysis. A total of 358 polymorphic bands were obtained from 39 of the 100 ISSR primers used, and 15 phenotypic traits were scored and used for genetic-similarity calculations and cluster analysis. The resulting dendrograms based on the ISSR and phenotypic data allowed almost all of the melon genotypes to be distinguished on the basis of the skin colour of the fruits. Mantel's test revealed a good correlation between the morphological and molecular data in their ability to detect genetic relationships among melon ecotypes (r = 0.50, P = 0.99). The data obtained confirm the effectiveness of this approach, and open new perspectives to reveal possible molecular associations with the phenotypic traits analysed.

Keywords

Cucumis meloinodorusinter-simple-sequence repeatlandracesphenotypic traits
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 214 - 217
Copyright
Copyright © NIAB 2011

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References

Danin-Poleg, Y, Tzuri, G, Reis, N and Katzir, N (1998) Application of inter-SSR markers in melon (Cucumis melo L.). Cucurbit Genetics Cooperative Report 21: 25–28.Google Scholar
Dhillon, NPS, Ranjana, R, Singh, K, Eduardo, I, Monforte, AJ, Pitrat, M, Dhillon, NK and Singh, PP (2007) Diversity among landraces of Indian snapmelon (Cucumis melo var. momordica). Genetic Resources and Crop Evolution 54: 1267–1283.Google Scholar
Ficcadenti, N, Sestili, S, Luongo, L, Campanelli, G, Rosa, A, Ribeca, C, Ferrari, V, Maestrelli, A, Genna, A and Belisario, A (2007) Recupero e valorizzazione di germoplasma di melone inodorus (Cucumis melo L.). Italus Hortus 14: 58–65.Google Scholar
Ficcadenti, N, Giardini, A, Sestili, S, Lo Scalzo, R, Palermo, ML, Tumbarello, B, Monteleone, G, Bono, M and Bongiovì, C (2010) Varietà migliorate e nuovi ibridi rilanciano il melone d'inverno. L'Informatore Agrario 9: 58–61.Google Scholar
Garcia, E, Jamilena, M, Alvarez, JI, Arnedo, T, Oliver, JL and Lozano, R (1998) Genetic relationships among melon breeding lines revealed by RAPD markers and agronomic traits. Theoretical and Applied Genetics 96: 878–885.Google Scholar
Gupta, M, Chyi, Y-S, Romero-Severson, J and Owen, JL (1994) Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats. Theoretical and Applied Genetics 89: 998–1006.Google Scholar
Levi, A and Thomas, CE (1999) An improved procedure for isolation of high quality DNA from watermelon and melon leaves. Cucurbit Genetics Cooperative Report 22: 41–42.Google Scholar
Lopez-Sesé, AI, Staub, JE and Gomez-Guillamon, ML (2003) Genetic analysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions. Theoretical and Applied Genetics 108: 41–52.Google Scholar
Mantel, M (1967) The detection of disease clustering and a generalized regression approach. Cancer Research 27: 209–220.Google Scholar
Nei, M and Li, W-H (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. The Proceedings of the National Academy of Sciences USA 76: 5269–5273.Google Scholar
Rohlf, FJ (2006) A comment on “phylogenetic correction”. Evolution 60: 1509–1515.CrossRefGoogle ScholarPubMed
Sestili, S, Minollini, A, Luciani, M, Campanelli, G and Ficcadenti, N (2004) Molecular characterization of several melon genotypes (Cucumis melo L.) by using molecular markers to identify resistance genes to Fusarium oxysporum f. sp. melonis. 15–18 September, XLVIII SIGA, Lecce, p. 182.Google Scholar
Sestili, S, Daniele, A, Rosa, A, Ferrari, V, Belisario, A and Ficcadenti, N (2008) Molecular characterization of different Italian inodorus melon populations based on ISSR molecular markers and preliminary SSR analysis. Proceedings of IX Eucarpia Meeting on Genetics and Breeding of Cucurbitaceae, 21–24 May, Avignon, France, pp. 307–311.Google Scholar
Staub, JE, Lopez-Sesè, AI and Fanourakis, N (2004) Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins. Euphytica 136: 151–166.Google Scholar
Stepanski, A, Kovalski, I and Perl-Treves, R (1999) Intra-specific classification of melons (Cucumis melo L.) in view of their phenotypic and molecular variation. Plant Systematics and Evolution 217: 313–332.Google Scholar
Van de Peer, Y (1994) TREECON for windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Computer Applications in the Biosciences 10: 569–570.Google ScholarPubMed