Hostname: page-component-848d4c4894-hfldf Total loading time: 0 Render date: 2024-05-23T00:38:50.262Z Has data issue: false hasContentIssue false
  • English
  • Français

Cuticular lesions: a non-infectious integumental disease of Glossina species

Published online by Cambridge University Press:  19 September 2011

M. O. Odindo ,
D. A. Turner ,
W. A. Otieno  and
G. P. Kaaya
M. O. Odindo
Affiliation:
ICIPE, Coastal Field Station, P.O. Box 80804, Mombasa, Kenya
D. A. Turner
Affiliation:
ICIPE, P.O. Box 30772, Nairobi, Kenya
W. A. Otieno
Affiliation:
ICIPE, Coastal Field Station, P.O. Box 80804, Mombasa, Kenya
G. P. Kaaya
Affiliation:
ICIPE, P.O. Box 30772, Nairobi, Kenya
Get access

Abstract

Purified suspensions of macerated cuticular lesions from the insectary-reared tsetse Glossina morsitans morsitans were applied on laboratory-reared newly emerged teneral tsetse by three pathways: topical application on the ventral abdominal integument, oral microfeeding, or microinjection into the haemocoel. A second group of flies were subjected to teratological wounding pin-stabs, forcep-pinch and shallow blade-slits, all in attempt to induce cuticular lesions. Samples of tsetse were examined every week for 11 weeks. Application of cuticular lesion suspensions did not result in lesion formation, but three types of lesions were formed due to physical damage induced artificially on the integument.

Total haemocyte counts (THCs) and differential haemocyte counts (DHCs) were also determined for tsetse with, and without, lesions. THCs were significantly higher (P < 0.01) in tsetse with cuticular lesions than in those without lesions. DHCs showed a high number of large spindle-shaped cells and thrombocytoids in the flies with lesions (P < 0.05).

Keywords

Glossina morsitans morsitanscuticular lesionsnon-infectious diseaseteratologytotal haemocyte countdifferential haemocyte countwound healing process
Type
Research Article
Information
International Journal of Tropical Insect Science , Volume 2 , Issue 4 , December 1981 , pp. 213 - 217
Copyright
Copyright © ICIPE 1981

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Adams, J. R., Stadelbacher, E. A. and Tompkins, G. J. (1979) A new virus-like particle isolated from the cotton bollworm, Heliothis zea. 37th Ann. Proc. Electron Microscopy Soc. Amer., San Antonio, Texas, pp. 52–53.Google Scholar
Gagen, S. J. and Ratcliffe, N. A. (1976) Studies on the in vitro cellular reactions and fate of injected bacteria in Galleria mellonella and Pieris brassicae larvae. J. Invertebr. Path. 28, 17–24.CrossRefGoogle Scholar
Jaenson, T. G. T. (1978) Virus-like rods associated with salivary gland hyperplasia in tsetse Glossina pallidipes. Trans. R. Soc. trop. Med. Hya. 11, 234–238.CrossRefGoogle Scholar
Kalmakoff, J., Williams, B. R. G. and Austin, F. J. (1977) Antiviral response in insects? J. Invertehr. Path. 29, 44–49.CrossRefGoogle ScholarPubMed
Murphy, F. A., Whitfield, S. G., Sudia, W. D. and Chamberlain, R. W. (1975) Interactions of vector with vertebrate pathogenic viruses. In Invertebrate Immunity—Mechanisms of Invertebrate Vector–Parasite Relations (Ed. by Maramorosch, K. and Shope, R. E.), pp. 25–48. Academic Press, New York.Google Scholar
Odindo, M. O., Sabwa, D. M., Amutalla, P. A. and Otieno, W. A. (1981) Preliminary tests on the transmission of virus-like particles to the tsetse Glossina pallidipes. Insect Sci. Application 2, 219–221.Google Scholar
Rowley, A. F. and Ratcliffe, N. A. (1978) A histological study of wound healing and haemocyte function in the wax-moth Galleria mellonella. J. Morph. 157, 181–199.CrossRefGoogle Scholar
Shapiro, M. (1968) Changes in the haemocyte population of the wax-moth. Galleria mellonella during wound healing. J. Insect. Physiol. 14, 1725–1733.CrossRefGoogle Scholar
Tanada, Y., Hukuhara, T. and Chang, G. Y. (1969) A strain of nuclear polyhedrosis virus causing extensive cellular hypertrophy. J. Invertebr. Path. 13, 394–409.CrossRefGoogle Scholar
Turnfer, D. A., Otieno, W. A. and Odindo, M. O. (1981) Cuticular lesions in tsetse flies (Glossinidae:Diptera) of Kenya. J. Invertebr. Path, (in press).Google Scholar
Watanabe, H. (1968) Abnormal cell proliferation in the epidermis of the fall webworm Hyphantrea eunea, induced by the infection of a nuclear-polyhedrosis virus. J. Invertebr. Path. 12, 310–320.CrossRefGoogle Scholar
Wigglesworth, V. B. (1973) Wound healing in an insect Rhodnius prolixus Hemiptera. J. exp. Biol. 14, 364–381.CrossRefGoogle Scholar
Zachary, D. and Hoffmann, J. A. (1973) The haemocytes of Calliphora erythroeephala Meig. (Diptera). Z. Zellforsch. mikrosk. Anat. 141, 55–73.CrossRefGoogle ScholarPubMed