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The effects of single and mixed infections of Apicystis bombi and deformed wing virus in Bombus terrestris

Published online by Cambridge University Press:  08 December 2015

PETER GRAYSTOCK Open the ORCID record for PETER GRAYSTOCK [Opens in a new window] ,
IVAN MEEUS ,
GUY SMAGGHE ,
DAVE GOULSON  and
WILLIAM O. H. HUGHES
PETER GRAYSTOCK*
Affiliation:
Institute of Integrative and Comparative Biology, University of Leeds, Leeds LS2 9JT, UK
IVAN MEEUS
Affiliation:
Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
GUY SMAGGHE
Affiliation:
Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
DAVE GOULSON
Affiliation:
School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK
WILLIAM O. H. HUGHES
Affiliation:
School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK
*
*Corresponding author. Institute of Integrative and Comparative Biology, University of Leeds, Leeds LS2 9JT, UK. E-mail: peter@graystock.info
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Summary

Many pollinators are currently suffering from declines, diminishing their gene pool and increasing their vulnerability to parasites. Recently, an increasing diversity of parasites has been recorded in bumblebees, yet for many, knowledge of their virulence and hence the risk their presence poses, is lacking. The deformed wing virus (DWV), known to be ubiquitous in honey bees, has now been detected in bumblebees. In addition, the neogregarine Apicystis bombi has been discovered to be more prevalent than previously thought. Here, we assess for the first time the lethal and sublethal effects of these parasites during single and mixed infections of worker bumblebees (Bombus terrestris). Fifteen days after experimental exposure, 22% of bees exposed to A. bombi, 50% of bees exposed to DWV and 86% of bees exposed to both parasites had died. Bumblebees that had ingested A. bombi had increased sucrose sensitivity (SS) and a lower lipid:body size ratio than control bees. While dual infected bumblebees showed no increase in SS. Overall, we find that A. bombi exhibits both lethal and sublethal effects. DWV causes lethal effect and may reduce the sub lethal effects imposed by A. bombi. The results show that both parasites have significant, negative effects on bumblebee health, making them potentially of conservation concern.

Keywords

pollinatoremerging diseasepathogenneogregarineDWV
Type
Research Article
Information
Parasitology , Volume 143 , Issue 3 , March 2016 , pp. 358 - 365
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Copyright
Copyright © Cambridge University Press 2015 

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