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Impact of mode of delivery on the milk microbiota composition of healthy women

Part of: The Gut Microbiome: How it is Shaped by Early Life

Published online by Cambridge University Press:  19 August 2015

R. Cabrera-Rubio ,
L. Mira-Pascual ,
A. Mira  and
M. C. Collado
R. Cabrera-Rubio
Affiliation:
Department of Biotechnology. Av. Agustin Escardino 7, Institute of Agrochemistry and Food Technology, Spanish National Research Council (IATA-CSIC), Valencia, Spain
L. Mira-Pascual
Affiliation:
Department of Biotechnology. Av. Agustin Escardino 7, Institute of Agrochemistry and Food Technology, Spanish National Research Council (IATA-CSIC), Valencia, Spain
A. Mira
Affiliation:
Department of Health and Genomics. Av. Cataluña 21, FISABIO Foundation, Center for Advanced Research in Public Health, Valencia, Spain
M. C. Collado*
Affiliation:
Department of Biotechnology. Av. Agustin Escardino 7, Institute of Agrochemistry and Food Technology, Spanish National Research Council (IATA-CSIC), Valencia, Spain
*
*Address for correspondence: M. C. Collado – IATA-CSIC, Av. Agustin Escardino 7, 49860 Paterna, Valencia, Spain. (Email mcolam@iata.csic.es)
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Abstract

Breast milk constitutes one of the most important sources of postnatal microbes. However, the influence of perinatal factors on the milk microbiome is still poorly understood. The aim of our study was to assess the impact of mode of delivery on the microbiome composition and diversity present in breast milk of healthy mothers. Mature milk samples (n=10) were taken from mothers after 1 month of exclusively breastfeeding. Microbiomes from milk samples were analyzed with 16S ribosomal RNA gene pyrosequencing and targeted quantitative polymerase chain reaction (PCR). Despite inter-individual variability in bacterial composition, The Principal Coordinates Analysis clearly separated milk microbiome from mothers with vaginal delivery (n=6) from those who undergo C-section (n=4). In addition, higher bacterial diversity and richness was found in milk samples from vaginal deliveries. Quantitative PCR data showed that higher levels of Bifidobacterium spp. were related significantly to lower levels of Staphylococcus spp. Despite the low sample size, our data suggest that mode of delivery has an important impact on milk microbiome composition. Further studies with larger sample sizes are needed to confirm these results and to understand the biological effects of C-section associated microbes on infant’s health.

Keywords

16S pyrosequencingC-sectiondiversitymilk microbiotaqPCRvaginal delivery
Type
Original Article
Information
Journal of Developmental Origins of Health and Disease , Volume 7 , Supplement 1: Themed Issue: The Gut Microbiome and Immunity: How it is Shaped by Early Life , February 2016 , pp. 54 - 60
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Copyright
© Cambridge University Press and the International Society for Developmental Origins of Health and Disease 2015 

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