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Fast Detection of Perkinsus Marinus, a Prevalent Pathogen of Oysters and Clams from Sea Waters

Published online by Cambridge University Press:  31 January 2011

Yu-Lin Wang ,
B.H. Chu ,
K.H. Chen ,
Chih-Yang Chang ,
Tanmay P. Lele ,
George Papadi ,
James K. Coleman ,
Barbara J. Sheppard ,
Chris F. Dungan  and
S. J. Pearton
...Show all authors
Yu-Lin Wang
Affiliation:
ylw59@ufl.edu, University of Florida, Chemical Engineering, Gainesville, Florida, United States
B.H. Chu
Affiliation:
ylin@che.ufl.edu, University of Florida, Chemical Engineering, Gainesville, Florida, United States
K.H. Chen
Affiliation:
nimochen@che.ufl.edu, University of Florida, Chemical Engineering, Gainesville, Florida, United States
Chih-Yang Chang
Affiliation:
chang.chihyang@gmail.com, United States
Tanmay P. Lele
Affiliation:
tlele@che.ufl.edu, University of Florida, Chemical Engineering, Gainesville, Florida, United States
George Papadi
Affiliation:
PapadiG@vetmed.ufl.edu, University of Florida, Infectious Diseases & Pathology, Gainesville, Florida, United States
James K. Coleman
Affiliation:
transfectz@hotmail.com, University of Florida, Infectious Diseases & Pathology, Gainesville, Florida, United States
Barbara J. Sheppard
Affiliation:
SheppardB@vetmed.ufl.edu, University of Florida, Infectious Diseases & Pathology, Gainesville, Florida, United States
Chris F. Dungan
Affiliation:
CDUNGAN@dnr.state.md.us, Cooperative Oxford Laboratory, Maryland Department of Natural Resources, Oxford, Maryland, United States
S. J. Pearton
Affiliation:
spear@mse.ufl.edu, Univ.Florida, Materials, PO Box 116400, Gainesville, Florida, 32611, United States
J.W. Johnson
Affiliation:
wjohnson@nitronex.com, Nitronex Corporation, Raleigh, North Carolina, United States
F. Ren
Affiliation:
ren@che.ufl.edu, University of Florida, Chemical Engineering, Gainesville, Florida, United States
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Abstract

Antibody-functionalized, Au-gated AlGaN/GaN high electron mobility transistors (HEMTs) were used to detect Perkinsus marinus. The antibody was anchored to the gate area through immobilized thioglycolic acid. The AlGaN/GaN HEMT were grown by a molecular beam epitaxy system (MBE) on sapphire substrates. Infected sea waters were taken from the tanks in which Tridacna crocea infected with P. marinus were living and dead. The AlGaN/GaN HEMT showed a rapid response of drain-source current in less than 5 seconds when the infected sea waters were added to the antibody-immobilized surface. The recyclability of the sensors with wash buffers between measurements was also explored. These results clearly demonstrate the promise of field-deployable electronic biological sensors based on AlGaN/GaN HEMTs for Perkinsus marinus detection.

Keywords

biologicalelectronic materialsensor
Type
Research Article
Information
MRS Online Proceedings Library (OPL) , Volume 1202: Symposium I – III-Nitride Materials for Sensing, Energy Conversion and Controlled Light-Matter Interactions , 2009 , 1202-I09-10
Copyright
Copyright © Materials Research Society 2010

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