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Molecular diversity in the Ukrainian melon collection as revealed by AFLPs and microsatellites

Published online by Cambridge University Press:  10 October 2008

Padmavathi Nimmakayala ,
Yan R. Tomason ,
Jooha Jeong ,
Gopinath Vajja ,
Amnon Levi ,
Paul Gibson  and
Umesh K. Reddy
Padmavathi Nimmakayala
Affiliation:
Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV25112, USA
Yan R. Tomason
Affiliation:
Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV25112, USA Department of Selection and Seed Production, Dnepropetrovsk State Agrarian University, Voroshilov 25, Dnepropetrovsk49600, Ukraine, e-mail: yantomason75@mail.ru
Jooha Jeong
Affiliation:
Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV25112, USA
Gopinath Vajja
Affiliation:
Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV25112, USA
Amnon Levi
Affiliation:
US Vegetable Laboratory, USDA, ARS, 2875 Savannah Highway, Charleston, SC29414, USA
Paul Gibson
Affiliation:
Department of Selection and Seed Production, Dnepropetrovsk State Agrarian University, Voroshilov 25, Dnepropetrovsk49600, Ukraine, e-mail: yantomason75@mail.ru Department of Plant, Soil and Agriculture Systems, Southern Illinois University, 62901Carbondale, IL, USA, e-mail: pgibson@siu.edu
Umesh K. Reddy*
Affiliation:
Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV25112, USA
*
*Corresponding author. E-mail: ureddy@wvstateu.edu
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Abstract

Thirty-eight melon accessions, which are of primary breeding importance in the Ukraine, were analysed for diversity. These collections represent a major non-US and non-western Europe source of melon germplasm that have not yet been subjected to molecular characterization. Molecular diversity was estimated based on a robust set of 465 polymorphisms gathered by amplified fragment length polymorphisms and simple sequence repeats (SSR). In this paper, we report 12 newly developed polymorphic SSR primer pairs, and their use for molecular characterization in the Ukrainian melon collections. Based on these polymorphisms, we estimated similarity indices that ranged from 0.70 to 1.00 among various accessions. The phylogenetic tree based on the similarity indices and a three-dimensional plot of the first three vectors of the principal component analysis corresponded fairly well with the existing three classical morphotypes namely aestivalis, europeus and hiemalis, under the convarEuropeus, which is also known as adana. The polymorphisms generated in the current study, which are specific to the grouping of fruit types and days to maturity will be very useful for further genetic studies and marker-assisted selections.

Keywords

AFLPCucumis melodiversitymelonSSR
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 2 , August 2009 , pp. 127 - 134
Copyright
Copyright © NIAB 2008

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References

Arumuganathan, K and Earle, ED (1991) Nuclear DNA content of some important plant species. Plant Molecular Biology Reporter 9: 208–218.CrossRefGoogle Scholar
Connell, JP, Pammi, S, Iqbal, MJ, Huizinga, T and Reddy, AS (1998) A high throughput procedure for capturing microsatellites from complex plant genomes. Plant Molecular Biology Reporter 16: 341–349.CrossRefGoogle Scholar
Danin-Poleg, Y, Reis, N, Tzuri, G and Katzir, N (2001) Development and characterization of microsatellite marker in Cucumis. Theoretical and Applied Genetics 102: 61–72.CrossRefGoogle Scholar
Decker-Walters, DS, Chung, S-M, Staub, JE, Quemada, HD and López- Sesé, AI (2002) The origin and genetic affinities of wild population of melon (Cucumis melo, Cucurbitaceae) in North America. Plant Systematics and Evolution 233: 183–197.CrossRefGoogle Scholar
Dhillon, NPS, Ranjana, R, Singh, Kuldeep, Eduardo, I, Monforte, AJ, Pitrat, M, Dhillon, NK and Singh, PP (2007) Diversity among landraces of India snapmelon (Cucumis melo var. momordica). Genetic Resources and Crop Evolution 54: 1267–1283.CrossRefGoogle Scholar
Felsenstein, J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.CrossRefGoogle ScholarPubMed
Garcia-Mas, J, Oliver, M, Gómez-Paniagua, H and De Vicente, MC (2000) Comparing AFLP, RAPD and FLP markers for measuring genetic diversity in melon. Theoretical and Applied Genetics 101: 860–864.CrossRefGoogle Scholar
Garcia-Mas, J, Monforte, AJ and Arús, P (2004) Phylogenetic relationships among Cucumis species based on the ribosomal internal transcribed spacer sequence and microsatellite markers. Plant Systematics and Evolution 248: 191–203.CrossRefGoogle Scholar
Gonzalo, MJ, Oliver, M, Garcia-Mas, J, Monfort, A, Dolcet-Sanjuan, R, Katzir, N, Arús, P and Monforte, AJ (2005) Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theoretical and Applied Genetics 110: 802–811.CrossRefGoogle ScholarPubMed
Greuter, W, Mcneil, J, Barrie, FR, Burdet, HM, Demoulin, V, Filgueiras, TS, Nicolson, DH, Silva, PC, Skog, JE, Trehane, P, Turland, NJ and Hawsworth, DL (eds), (2002) The International Code of Botanical Nomenclature (Saint Louis Code) Sixteenth International Botanical Congress. St Louis, MS, July–August 1999 Regnum Vegetabile 138: 1–474. Königstein, Germany: Koeltz Scientific Books.Google Scholar
Jaccard, P (1908) Nouvelles rescherches sur la distribution florale. Bulletin Société Vaudoise des Sciences Naturelles 44: 223–270.Google Scholar
López-Sesé, AI, Staub, JE, Katzir, N and Gómez-Guillamón, ML (2002) Estimation of between and within accession variation in selected Spanish melon germplasm using RAPD and SSR markers to assess strategies for large collection evaluation. Euphytica 127: 41–51.CrossRefGoogle Scholar
López-Sesé, AI, Staub, JE and Gómez-Guillamón, ML (2003) Genetic analysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions. Theoretical and Applied Genetics 108: 41–52.CrossRefGoogle ScholarPubMed
Mliki, A, Staub, JE, Zhangyong, S and Ghorbel, A (2001) Genetic diversity in melon (Cucumis melo L.): an evaluation of African germplasm. Genetic Resources and Crop Evolution 48: 587–597.CrossRefGoogle Scholar
Monforte, AJ, Garcia-Mas, J and Arús, P (2003) Genetic variability in melon based on microsatellite variation. Plant breeding 122: 153–157.CrossRefGoogle Scholar
Munger, HM and Robinson, RW (1991) Nomenclature of Cucumis melo L. Cucurbit Genetics Cooperative Report 14: 43–44.Google Scholar
Nakata, Eijiro, Staub, JE, López-Sesé, AI and Katzir, N (2005) Genetic diversity of Japanese melon cultivars (Cucumis melo L.) as assessed by random amplified polymorphic DNA and simple sequence repeat markers. Genetic Resources and Crop Evolution 52: 405–419.CrossRefGoogle Scholar
Oliver, M, Garcia-Mas, J, Morales, M, Dolcet-Sanjuan, R, Vicente, M, Gómez, H, Leeuwen, H, Monfort, A, Puigdomenech, P and Arús, P (2000) Proceedings of Cucurbitaceae 2000. In: Katzir, N and Paris, HS (eds) VII Eucarpia Meeting on Cucurbit Genetics and Breeding Acta Horticulturae. Israel: Ma'ale Ha Hamisha, pp. 375–378.Google Scholar
Pangalo, KI (1958) Melons [Dyni]. Kishinev (MD): State publisher [Gosudarstvennoe izdatel'stvo], pp. 350–433.Google Scholar
Périn, C, Hagen, L, De Conto, V, Katzir, N, Danin-Poleg, Y, Portnoy, V, Baudracco-Arnas, S, Chadoeuf, J, Dogimont, C and Pitrat, M (2002) A reference map of Cucumis melo based on two recombinant inbred line populations. Theoretical and Applied Genetics 104: 1017–1034.CrossRefGoogle ScholarPubMed
Pyzhenkov, VI and Malinina, MI (1994) Cucurbits (cucumber, melon). Flora of Cultivated Plants XXI. Moscow: Kolos, pp. 200–288.Google Scholar
Reddy, OUK, Pepper, AE, Abdurakhmonov, I, Saha, S, Jenkins, JN, Brooks, T and BoelkYEl-Zik, K (2001) New di-nucleotide and tri-nucleotide micorsatellite marker resources for cotton genome research. Journal of Cotton Science 5: 103–113.Google Scholar
Ritschel, PS, De Lima Lins, TC, Tristan, RL, Cortopassi Buso, GS, Buso, JA and Ferrira, ME (2004) Development of microsatellite markers from an enriched genomic library for genetic analysis of melon (Cucumis melo L.). BMC Plant Biology 4: 1471–2229.CrossRefGoogle ScholarPubMed
Robinson, RW and Decker-Walters, DS (1997) Cucurbits. New York: CAB International, pp. 156–200.Google Scholar
Rohlf, FJ (1998) NTSYS-pc: numerical taxonomy and multivariate analysis system. Program Manual. New York: Exeter Software.Google Scholar
Saitou, N and Nei, M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406–425.Google Scholar
Sensoy, S, Büyükalaca, S and Abak, K (2007) Evaluation of genetic diversity in Turkish melons (Cucumis melo L.) based on phenotypic characters and RAPD markers. Genetic Resources and Crop Evolution 54: 1351–1365.CrossRefGoogle Scholar
Silberstein, L, Kovalski, I, Ruguo, Huang, Anagnostou, K, Jahn, MMK and Perl-Treves, R (1999) Molecular variation in melo (Cucumis melo L.) as revealed by RFLP and RAPD markers. Scientia Horticulturae 79: 101–111.CrossRefGoogle Scholar
Staub, JE, Danin-Poleg, Y, Fazio, G, Horejsi, T, Reis, N and Katzir, N (2000) Comparative analysis of cultivated melon groups (Cucumis melo L.) using random amplified polymorphic DNA and simple sequence repeat markers. Euphytica 115: 225–241.CrossRefGoogle Scholar
Staub, JE, López-Sesé, AI and Fanourakis, N (2004) Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins. Euphytica 136: 151–166.CrossRefGoogle Scholar
Stepansky, A, Kovalski, I and Perl-Treves, R (1999) Intraspecific classification of melon (Cucumis melo L.) in view of their phenotypic and molecular variation. Plant Systematics and Evolution 217: 313–332.CrossRefGoogle Scholar
Szabó, Z, Gyulai, G, Humphreys, M, Horváth, L, Bittsánszky, A, Lágler, R and Heszky, L (2005) Genetic variation of melon (Cucumis melo L.) compared to an extinct landrace from the middle ages (Hungry) 1. rDNA, SSR and SNP analysis of 47 cultivars. Euphytica 146: 87–94.CrossRefGoogle Scholar
Tanaka, K, Nishitani, A, Akashi, Y, Sakata, Y, Nishida, H, Yoshino, H and Kato, K (2007) Molecular characterization of South and East Asian melon, Cucumis melo L., and the origin of Group Conomon var. makuwa and var. conomon revealed by RAPD analysis. Euphytica 153: 233–247.CrossRefGoogle Scholar
Tomason, YR (2002) Selection of melon lines with female flower type and morphological markers to create heterotic F1 hybrids PhD Thesis, Dnepropetrovsk State Agrarian University.Google Scholar
Urina, OV, Pivovarov, VF and Balashova, NN (1998) Breeding and Seed Production of Cucurbit Crops in Russia. Moscow: VNIISSOK, pp. 400–410.Google Scholar
Vos, P, Hogers, R, Bleeker, M, Reijans, M, Van de Lee, T, Hornes, M, Fritjers, A, Pot, J, Peleman, J, Kuiper, M and Zabeau, M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407–4414.CrossRefGoogle ScholarPubMed
Yashiro, K, Iwata, H, Akashi, Y, Tomita, K, Kuzuya, M, Tsumura, Y and Kato, K (2005) Genetic relationship among East and South Asian melon (Cucumis melo L.) revealed by AFLP analysis. Breeding Science 55: 197–206.CrossRefGoogle Scholar
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