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Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm

Published online by Cambridge University Press:  08 April 2013

Marc Suquet ,
Claudie Quere ,
Christian Mingant ,
Luc Lebrun ,
Dominique Ratiskol ,
Philippe Miner  and
Jacky Cosson
Marc Suquet*
Affiliation:
Ifremer, UMR 6539, Physiologie fonctionnelle des organismes marins, Station expérimentale d’Argenton, Argenton, France
Claudie Quere
Affiliation:
Ifremer, UMR 6539, Physiologie fonctionnelle des organismes marins, Plouzané, France
Christian Mingant
Affiliation:
Ifremer, UMR 6539, Physiologie fonctionnelle des organismes marins, Station expérimentale d’Argenton, Argenton, France
Luc Lebrun
Affiliation:
Ifremer, UMR 6539, Physiologie fonctionnelle des organismes marins, Station expérimentale d’Argenton, Argenton, France
Dominique Ratiskol
Affiliation:
Ifremer, UMR 6539, Physiologie fonctionnelle des organismes marins, Station expérimentale d’Argenton, Argenton, France
Philippe Miner
Affiliation:
Ifremer, UMR 6539, Physiologie fonctionnelle des organismes marins, Plouzané, France
Jacky Cosson
Affiliation:
University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, 38925 Vodnany, Czech Republic
*
a Corresponding author: marc.suquet@ifremer.fr
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Abstract

Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (VAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, VAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed after serotonin injection. From the distal part of testes up to the gonopore, an increase of the percentage of motile spermatozoa and VAP was assessed, suggesting a sperm ‘maturation process’ along the genital ducts. A higher increase in the percentage of motile sperm was recorded during a 5 min incubation of testicular sperm in seawater containing 2 mM serotonin and seawater containing 10 mM caffein compared to seawater (control). In addition, a higher VAP was assessed, incubating testicular sperm in caffein, compared to control or serotonin. Then, the percentage of motile spermatozoa, VAP and intracellular ATP content exhibited a progressive reduction during the 10 h swimming period. Mean values of the percentage of motile spermatozoa, VAP, sperm track linearity (LIN) and intracellular ATP content recorded at the beginning of the movement period for sperm samples collected after intragonadal serotonin injection, were 82 ± 7%, 162 ± 15μm s-1, 0.33 ± 0.12 and 212 ± 133 nmol  × 10-9 spermatozoa (n = 9 males), respectively. The present study confirms the existence of a sperm “maturation process” along scallop genital ducts. In addition, the cessation of scallop sperm movement can be explained by the exhaustion of ATP content at the end of the movement phase.

Keywords

SpermatozoaCASAMaturationSperm motilityBivalvePecten maximus
Type
Research Article
Information
Aquatic Living Resources , Volume 26 , Issue 3 , 2013 , pp. 215 - 220
Copyright
© EDP Sciences, IFREMER, IRD 2013

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