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The effects of extracellular ATP and its receptor antagonists on pig oocytes during in vitro maturation

Published online by Cambridge University Press:  02 December 2014

Erika Wakizoe ,
Koji Ashizawa ,
Shinsuke H. Sakamoto ,
Koichiro Hemmi ,
Ikuo Kobayashi  and
Yasuhiro Tsuzuki
Erika Wakizoe
Affiliation:
Animal Reproduction Laboratory, Faculty of Agriculture, University of Miyazaki, Miyazaki 889–2192, Japan.
Koji Ashizawa
Affiliation:
Animal Reproduction Laboratory, Faculty of Agriculture, University of Miyazaki, Miyazaki 889–2192, Japan.
Shinsuke H. Sakamoto
Affiliation:
Division of Bio-Resources, Frontier Research Center, University of Miyazaki889–1692, Japan.
Koichiro Hemmi
Affiliation:
Sumiyoshi Livestock Station, Field Science Center, Faculty of Agriculture, University of Miyazaki, Miyazaki 880–0121, Japan.
Ikuo Kobayashi
Affiliation:
Sumiyoshi Livestock Station, Field Science Center, Faculty of Agriculture, University of Miyazaki, Miyazaki 880–0121, Japan.
Yasuhiro Tsuzuki*
Affiliation:
Animal Reproduction Laboratory, Faculty of Agriculture, University of Miyazaki, Miyazaki 889–2192, Japan. Animal Reproduction Laboratory, Faculty of Agriculture, University of Miyazaki, Miyazaki 889–2192, Japan.
*
All correspondence to: Yasuhiro Tsuzuki. Animal Reproduction Laboratory, Faculty of Agriculture, University of Miyazaki, Miyazaki 889–2192, Japan. e-mail: a01207u@cc.miyazaki-u.ac.jp
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Summary

We measured the ATP concentrations in the porcine follicular fluid derived from three sizes of follicles (small: <3 mm, medium: 3–6 mm, large: >6 mm in diameter). Then, the effects of pre-treatment (100 μM each for 30 min before maturation) with antagonists for extracellular ATP receptor P2X or P2Y on the nuclear maturation rate of cumulus-cell-enclosed (COs) or -denuded oocytes (DOs) up to the preovulatory stage in the presence or absence of 20 nM ATP (a similar concentration to that of medium-sized follicle fluid) were investigated. The antagonists used were pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid (PPADS) or reactive blue 2 (RB2), for extracellular ATP receptor P2X and P2Y, respectively. In addition, the embryonic development rates of COs pre-treated with RB2 were also evaluated. It was found that when the follicular sizes increased, the ATP concentrations significantly decreased (P < 0.05). No differences were observed in the nuclear maturation rates among all COs, regardless of pre-treatment with (+) or without (–) PPADS and in the presence (+) or absence (–) of ATP during maturation. In contrast, the nuclear maturation rate of the COs, but not DOs, in the ATP(–) RB2(+) group was significantly lower (P < 0.05) than that of the ATP(–) RB2(–) and ATP(+)RB2(–) groups. The pronuclear formation and blastocyst formation rates by parthenogenetic activation in the ATP(–) RB2(+) and ATP(+) RB2(+) groups were significantly lower (P < 0.05) than those in the ATP(–) RB2(–) group. In conclusion, it is suggested that the nuclear maturation of porcine oocytes may be influenced by the ATP receptor P2Y present in the cumulus cells.

Keywords

AntagonistExtracellular ATPIn vitro maturationPorcine oocytesP2Y receptor
Type
Research Article
Information
Zygote , Volume 23 , Issue 6 , December 2015 , pp. 885 - 892
Copyright
Copyright © Cambridge University Press 2014 

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