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Involvement of cAMP and calmodulin in endocytic yolk uptake during Xenopus laevis oogenesis

Published online by Cambridge University Press:  04 May 2011

Melchor Emilio Luque ,
María de los Angeles Serrano ,
María Eugenia Mónaco ,
Evelina Inés Villecco  and
Sara Serafina Sánchez
Melchor Emilio Luque
Affiliation:
Instituto Superior de Investigaciones Biológicas (CONICET), Universidad Nacional de Tucumán, Departamento de Biología del Desarrollo, San Miguel de Tucumán
María de los Angeles Serrano
Affiliation:
Instituto Superior de Investigaciones Biológicas (CONICET), Universidad Nacional de Tucumán, Departamento de Biología del Desarrollo, San Miguel de Tucumán
María Eugenia Mónaco
Affiliation:
Instituto Superior de Investigaciones Biológicas (CONICET), Universidad Nacional de Tucumán, Departamento de Biología del Desarrollo, San Miguel de Tucumán
Evelina Inés Villecco
Affiliation:
Instituto Superior de Investigaciones Biológicas (CONICET), Universidad Nacional de Tucumán, Departamento de Biología del Desarrollo, San Miguel de Tucumán
Sara Serafina Sánchez*
Affiliation:
Instituto Superior de Investigaciones Biológicas (CONICET), Universidad Nacional de Tucumán, Departamento de Biología del Desarrollo, Chacabuco 461, T4000ILI San Miguel de Tucumán, Argentina.
*
All correspondence to: Sara Serafina Sánchez. Instituto Superior de Investigaciones Biológicas (CONICET), Universidad Nacional de Tucumán, Departamento de Biología del Desarrollo, Chacabuco 461, T4000ILI San Miguel de Tucumán, Argentina. Tel: +54 381 4107214. Fax: +54 381 4247752. e-mail: ssanchez@fbqf.unt.edu.ar
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Summary

The aim of the present study was to show the participation and physiological role of calmodulin (CaM) and cAMP during vitellogenin endocytic uptake in the amphibian Xenopus laevis. The results showed a differential distribution of CaM in the ovary follicles during oogenesis. The CaM intracellular localization was not affected by gap junction's downregulation and CaM inhibition did not completely abolished the endocytic activity of oocytes. We showed that cAMP was able to completely rescue the endocytic competence in follicles in which gap junctional communication had been disrupted by octanol. Moreover cAMP was capable of restoring oocyte endocytic capability in the presence of octanol and stelazine, a CaM inhibitor. We propose that, in Vtg uptake regulation, cAMP is upstream of CaM during the endocytic signalling pathway.

Keywords

CalmodulincAMPSignalling pathwaysVitellogenesisXenopus laevis
Type
Research Article
Information
Zygote , Volume 21 , Issue 1 , February 2013 , pp. 1 - 9
Copyright
Copyright © Cambridge University Press 2011

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