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Heparin effect on in vitro nuclear maturation of bovine oocytes

Published online by Cambridge University Press:  01 February 2008

Juan Carlos Flores-Alonso ,
Leticia Lezama-Monfil ,
María Luisa Sánchez-Vázquez ,
Rosalina Reyes  and
Néstor M. Delgado
Juan Carlos Flores-Alonso
Affiliation:
Biology of Reproduction Laboratory, East Biomedical Research Center, General Zone Hospital #5, Mexican Institute of Social Security, Puebla, Mexico
Leticia Lezama-Monfil
Affiliation:
Biology of Reproduction Laboratory, East Biomedical Research Center, General Zone Hospital #5, Mexican Institute of Social Security, Puebla, Mexico
María Luisa Sánchez-Vázquez
Affiliation:
Biology of Reproduction Laboratory, East Biomedical Research Center, General Zone Hospital #5, Mexican Institute of Social Security, Puebla, Mexico
Rosalina Reyes
Affiliation:
Biology of Reproduction Laboratory, East Biomedical Research Center, General Zone Hospital #5, Mexican Institute of Social Security, Puebla, Mexico
Néstor M. Delgado*
Affiliation:
Biology of Reproduction Laboratory, East Biomedical Research Center, General Zone Hospital #5, Mexican Institute of Social Security, Puebla, Mexico
*
†All correspondence to: Juan Carlos Flores-Alonso, Biology of Reproduction Laboratory, East Biomedical Research Center, Benito Juárez 1915 Colonia Guadalupe Victoria. C. P. 74280. Atlixco, Puebla, Mexico. Tel: +244 44 401 22 or +244 447 20 46; Fax: +222 2 36 35 27; e-mail: jcarfa@prodigy.net.mx
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Summary

Oocytes undergo numerous biochemical and morphological changes during their development from preantral to preovulatory phases. In vitro studies have suggested several compounds that might induce oocyte maturation. Heparin is a natural component of ooplasm, follicular fluid and uterine fluid and previous studies indicated that it might act as a chromatin maturation factor in bovine oocytes. We tested this hypothesis in vitro by timing germinal vesicle breakdown (GVBD) and first polar body (PB) formation without any other natural or introduced factors that might influence the rate of oocyte maturation. We also determined if these oocytes could be fertilized.

Bovine oocytes were incubated in a salt medium and TCM 199 supplemented with different concentrations of heparin for 24 h at 37.5 °C in a humidified atmosphere of 5% CO2. With 1.0 and 6.5 mg/ml heparin, the time of GVBD was reduced from 4.7 ± 1.1 h to about 1.5 h and the time of first PB formation was reduced from 22.0 ± 1.1 h to 9.0–11.0 h in salt medium. In TCM 199, only 6.5 mg/ml heparin significantly reduced the time of PB formation. In both incubation media, 1.0 and 6.5 mg/ml heparin induced GVBD, extrusion of the first PB and formation of the metaphase II nucleus. Moreover, heparin did not interfere with the fertilization of oocytes matured in TCM 199. Based on the results, we propose that heparin plays an important role in the rearrangement of the oocyte chromatin and acts as an oocyte maturation factor.

Keywords

Chromatin reorganizationGerminal vesicle breakdownHeparinOocyte maturationPolar body formation
Type
Research Article
Information
Zygote , Volume 16 , Issue 1 , February 2008 , pp. 1 - 8
Copyright
Copyright © Cambridge University Press 2008

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