Hostname: page-component-8448b6f56d-gtxcr Total loading time: 0 Render date: 2024-04-17T21:13:13.497Z Has data issue: false hasContentIssue false
  • English
  • Français

Murine Asb-17 expression during mouse testis development and spermatogenesis

Published online by Cambridge University Press:  09 August 2004

Kye-Seong Kim ,
Myung-Sun Kim ,
Soo-Kyung Kim  and
Kwang-Hyun Baek
Kye-Seong Kim
Affiliation:
Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul, Korea
Myung-Sun Kim
Affiliation:
Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul, Korea
Soo-Kyung Kim
Affiliation:
Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul, Korea
Kwang-Hyun Baek
Affiliation:
Cell and Gene Therapy Research Institute, Graduate School of Life Science and Biotechnology, Pochon CHA University, CHA General Hospital, Seoul, Korea
Get access Rights & Permissions [Opens in a new window]

Abstract

In this study we isolated a murine mAsb-17 from mouse testis by RT-PCR using primers designed based on the sequences from the GenBank database. The sequence analysis showed that mAsb-17 encodes a 295 amino acid polypeptide with a molecular weight of approximately 34 kDa containing two ankyrin repeats and one SOCS box. The amino acid sequence of mASB-17 showed 87.5%, 98.3% and 92.9% identity with that of human, rat and dog, respectively. Interestingly, northern blot analysis showed that mAsb-17 was expressed only in the testis. The expression analysis by RT-PCR for mAsb-17 in mouse indicates that mAsb-17 is expressed from the fourth week after birth to adult, with the highest expression in round spermatids. Both northern blot and RT-PCR analyses suggest that mASB-17 may play essential roles in testis development and spermatogenesis.

Keywords

Ankyrin repeatAsb-17SOCS boxSpermatogenesis
Type
Research Article
Information
Zygote , Volume 12 , Issue 2 , May 2004 , pp. 151 - 156
Copyright
2004 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)