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Molecular characterisation of Beauveria bassiana isolates obtained from overwintering sites of Sunn Pests (Eurygaster and Aelia species)

Published online by Cambridge University Press:  24 March 2005

Marilena AQUINO DE MURO ,
Sarah ELLIOTT ,
David MOORE ,
Bruce L. PARKER ,
Margaret SKINNER ,
William REID  and
Mustapha EL BOUHSSINI
Marilena AQUINO DE MURO
Affiliation:
CABI Bioscience UK Centre, Bakeham Lane, Egham TW20 9TY, UK.
Sarah ELLIOTT
Affiliation:
CABI Bioscience UK Centre, Bakeham Lane, Egham TW20 9TY, UK.
David MOORE
Affiliation:
CABI Bioscience UK Centre, Bakeham Lane, Egham TW20 9TY, UK.
Bruce L. PARKER
Affiliation:
University of Vermont, 661 Spear Street, Burlington, VT 05401, USA.
Margaret SKINNER
Affiliation:
University of Vermont, 661 Spear Street, Burlington, VT 05401, USA.
William REID
Affiliation:
University of Vermont, 661 Spear Street, Burlington, VT 05401, USA.
Mustapha EL BOUHSSINI
Affiliation:
ICARDA, P.O. Box 5466, Aleppo, Syria. E-mail: m.demuro@cabi.org
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Abstract

110 isolates of Beauveria (104 B. bassiana, 5 Beauveria spp., 1 B. brongniartii) were obtained from Sunn Pests (Eurygaster and Aelia species), litter, and other insect samples at overwintering sites in seven countries in the Middle East and West Asia. DNA was extracted from these isolates, and four techniques were used to characterize and to investigate genetic diversity at the molecular level: ITS-RFLP, ITS sequencing, ISSR-PCR, and AFLP. The ITS-RFLP and ITS sequences did not detect significant genetic variation among the isolates. However, both ISSR-PCR and AFLP analyses gave indications of intraspecific groupings correlated with geographical origin and relative genetic diversity among some isolates, but no obvious association with Sunn Pest hosts. There was no obvious genotypic grouping of B. bassiana isolates from E. integriceps, perhaps suggesting the overwintering populations were infected by generalist native isolates rather than by host-specific ones that might be more suitable for biocontrol purposes.

Type
Research Article
Information
Mycological Research , Volume 109 , Issue 3 , March 2005 , pp. 294 - 306
Copyright
© The British Mycological Society 2005

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