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The serological grouping system for Serpulina (Treponema) hyodysenteriae

Published online by Cambridge University Press:  15 May 2009

T. T. A. Lau  and
D. J. Hampson
T. T. A. Lau
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia, 6150
D. J. Hampson*
Affiliation:
School of Veterinary Studies, Murdoch University, Perth, Western Australia, 6150
*
* To whom all correspondence should be addressed.
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Summary

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Lipopolysaceharide from serostrains of Serpulina (Treponema) hyodysenteriae for serogroups A to I was characterized using sodium dodecylsulphate polyacrylamide gel electrophoresis and silver staining. All strains had lipo-polysaccharide components ranging from 10 to 16 kDa that represented lipid A-core polysaccharide regions, and short O-antigen side chain were also recognized in certain immunoblots.

Serological reactions between lipopolysaceharide and antisera against each of these serostrains were examined by Western immunoblotting. There was relatively little antigenic cross-reactivity between LPS from the nine strains, thus confirming their suitability as serostrains.

Using cross-absorbed sera, isolates within serogroups A and E were shown to possess unique epitopes on the core lipopolysaceharide, distinct from serogroup reactivities. These isolates were therefore identified as serovars within the serogroups.

This study confirmed the usefulness of the serotyping scheme for S. hyodysenteriae, in which the bacteria can be placed into serogroups using unabsorbed sera, and into serovars within these using cross-absorbed sera.

Type
Research Article
Information
Epidemiology & Infection , Volume 109 , Issue 2 , October 1992 , pp. 255 - 263
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.Stanton, TB, Lebo, DF. Treponema hyodysenteriae growth under various culture conditions. Vet Microbiol 1988; 24: 177–90.Google Scholar
2.Stanton, TB, Jensen, NS, Casey, TA, Tordoff, LA, Dewhirst, FE, Paster, BJ. Reclassification of Treponema hyodysenteriae and Treponema innocens in a new genus Serpula gen. nov. as Serpula hyodysenteriae comb. nov. and Serpula innocens comb. nov. Int J System Bacteriol 1991; 41: 50–8.CrossRefGoogle Scholar
3.Stanton, TB. Proposal to change the genus designation Serpula to Serpulina gen. nov. containing the species Serpulina hyodysenteriae comb. nov. and Serpulina innocens comb. nov. Int J System Bacteriol 1992; 42: 189–92.Google Scholar
4.Taylor, DJ, Alexander, TJL. The production of dysentery in swine by feeding cultures containing a spirochaete. Br Vet J 1971; 127: 58–61.CrossRefGoogle ScholarPubMed
5.Harris, DL, Glock, RD, Christensen, CR, Kinyon, JM. Swine dysentery. I. Inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction of the disease. Vet Med Small Animal Clinician 1972; 67: 61–4.Google Scholar
6.Luderitz, O, Freundenberg, MA, Galanos, C, Lehmann, V, Riestchel, ETH, Shaw, DH. Lipopolysaccharides of Gram negative bacteria. Curr Top Memb Transp 1982; 17: 79–151.CrossRefGoogle Scholar
7.Baum, DH, Joens, LA. Serotypes of beta-haemolytic Treponema hyodysenteriae. Infect Immun 1979; 25: 792–7.CrossRefGoogle ScholarPubMed
8.Lemcke, RM, Bew, J. Antigenic differences among isolates of Treponema hyodysenteriae. Proc Int Pig Vet Soc Cong Ghent. 1984; 183.Google Scholar
9.Mapother, ME, Joens, LA. New serotypes of Treponema hyodysenteriae. J Clin Microbiol 1985; 22: 161–4.CrossRefGoogle ScholarPubMed
10.Hampson, DJ, Mhoma, JRL, Combs, BG, Buddle, JR. Proposed revisions to the serological typing system for Treponema hyodysenteriae. Epidemiol Infect 1989; 102: 75–84.Google Scholar
11.Hampson, DJ, Mhoma, JRL, Combs, BG, Lee, JI. Serological grouping of Treponema hyodysenteriae. Epidemiol Infect 1990; 105: 79–85.CrossRefGoogle ScholarPubMed
12.Hampson, DJ. New serogroups of Treponema hyodysenteriae (G H and I). Vet Rec 1990; 127: 524.Google Scholar
13.Li, Z, Belanger, M, Jacques, M. Serotyping of Canadian isolates of Treponema hyodysenteriae and description of two new serotypes, J Clin Microbiol 1991; 29: 2794–7.Google Scholar
14.Hampson, DJ, Mhoma, JRL, Combs, BG. Analysis of lipopolysaccharide antigens of Treponema hyodysenteriae. Epidemiol Infect 1989; 103: 275–84.Google Scholar
15.Tsai, CM, Frasch, CE. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Analyt Biochem 1982; 119: 115–19.CrossRefGoogle ScholarPubMed
16.Chatfield, SN, Fernie, DS, Penn, C, Dougan, G. Identification of the major antigens of Treponema hyodysenteriae and comparison with those of Treponema innocens. Infect Immun 1988; 56: 1070–5.Google Scholar
17.Wannemuehler, MJ, Hubbard, RD, Greer, JM. Characterization of the major outer membrane antigens of Treponema hyodysenteriae. Infect Immun 1988; 56: 3032–9.CrossRefGoogle ScholarPubMed
18.Halter, MR, Joens, LA. Lipooligosaccharides from Treponema hyodysenteriae and Treponema innocens. Infect Immun 1988; 56: 3152–6.Google Scholar
19.Jann, B, Konrad, R, Jann, K. Heterogeneity of lipopolysaccharides. Analysis of poly-saccharide chain lengths by sodium dodecylsulphate-polyacrylamide gel electrophoresis. Eur J Biochem 1975; 60: 239–46.Google Scholar
20.Hitchcock, PJ, Brown, TM. Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 1983; 154: 269–77.Google Scholar
21.Palva, ET, Makela, PH. Lipopolysaccharide heterogeneity in Salmonella typhimurium analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Eur J Biochem 1980; 107: 137–43.CrossRefGoogle Scholar
22.Morrison, DC, Leive, L. Fractions of lipopolysaccharide from Escherichia coli 0111: B4 prepared by two extraction procedures. J Biol Chem 1975; 250: 2911–19.CrossRefGoogle Scholar