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Single-molecule fluorescence to study molecular motors

Published online by Cambridge University Press:  31 July 2007

Hyokeun Park ,
Erdal Toprak  and
Paul R. Selvin
Hyokeun Park
Affiliation:
Department of Chemistry, University of Illinois, Urbana, IL, USA
Erdal Toprak
Affiliation:
Center for Biophysics and Computational Biology, University of Illinois, Urbana, IL, USA
Paul R. Selvin*
Affiliation:
Center for Biophysics and Computational Biology, University of Illinois, Urbana, IL, USA Department of Physics, University of Illinois, Urbana, IL, USA
*
*Author for correspondence: Dr P. R. Selvin, Physics Department, 1110 West Green Street, Loomis Laboratory, University of Illinois, Urbana, IL 61801, USA. Tel.: 217-244-3371; Fax: 217-244-7559; E-mail: selvin@uiuc.edu
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Abstract

Molecular motors, which use energy from ATP hydrolysis to take nanometer-scale steps with run-lengths on the order of micrometers, have important roles in areas such as transport and mitosis in living organisms. New techniques have recently been developed to measure these small movements at the single-molecule level. In particular, fluorescence imaging has contributed to the accurate measurement of this tiny movement. We introduce three single-molecule fluorescence imaging techniques which can find the position of a fluorophore with accuracy in the range of a few nanometers. These techniques are named after Hollywood animation characters: Fluorescence Imaging with One Nanometer Accuracy (FIONA), Single-molecule High-REsolution Colocalization (SHREC), and Defocused Orientation and Position Imaging (DOPI). We explain new understanding of molecular motors obtained from measurements using these techniques.

Type
Review Article
Information
Quarterly Reviews of Biophysics , Volume 40 , Issue 1 , February 2007 , pp. 87 - 111
Copyright
Copyright © Cambridge University Press 2007

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