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BnSP-7 toxin, a basic phospholipase A2 from Bothrops pauloensis snake venom, interferes with proliferation, ultrastructure and infectivity of Leishmania (Leishmania) amazonensis

Published online by Cambridge University Press:  27 February 2013

DÉBORA C. O. NUNES ,
MÁRCIA M. N. R. FIGUEIRA ,
DAIANA S. LOPES ,
DAYANE L. NAVES DE SOUZA ,
LUIZ FERNANDO M. IZIDORO ,
ELOÍSA A. V. FERRO ,
MARIA A. SOUZA ,
RENATA S. RODRIGUES ,
VERIDIANA M. RODRIGUES  and
KELLY A. G. YONEYAMA
DÉBORA C. O. NUNES
Affiliation:
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil
MÁRCIA M. N. R. FIGUEIRA
Affiliation:
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil
DAIANA S. LOPES
Affiliation:
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil
DAYANE L. NAVES DE SOUZA
Affiliation:
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil
LUIZ FERNANDO M. IZIDORO
Affiliation:
Faculdade de Ciências Integradas do Pontal, Universidade Federal de Uberlândia, MG, Brazil
ELOÍSA A. V. FERRO
Affiliation:
Departamento de Morfologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brazil
MARIA A. SOUZA
Affiliation:
Departamento de Patologia, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, MG, Brazil
RENATA S. RODRIGUES
Affiliation:
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica (N-Biofar), 31270-901, Belo Horizonte, MG, Brazil
VERIDIANA M. RODRIGUES
Affiliation:
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica (N-Biofar), 31270-901, Belo Horizonte, MG, Brazil
KELLY A. G. YONEYAMA*
Affiliation:
Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, UFU, Uberlândia, MG, Brazil
*
*Corresponding author: Pará avenue, 1720 CEP: 38400-902 Uberlândia, MG, Brazil. Tel: +3432182203. Fax: +55 34 3218 2203#22. E-mail: kellyagy@ingeb.ufu.br
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Summary

This paper reports the effects of BnSP-7 toxin, a catalytically inactive phospholipase A2 from Bothrops pauloensis snake venom, on Leishmania (Leishmania) amazonensis. BnSP-7 presented activity against promastigote parasite forms both in the MTT assay, with IC50 of 58·7 μg mL−1 of toxin, and a growth curve, inhibiting parasite proliferation 60–70% at concentrations of 50–200 μg mL−1 of toxin 96 h after treatment. Also, the toxin presented effects on amastigotes, reducing parasite viability by 50% at 28·1 μg mL−1 and delaying the amastigote–promastigote differentiation process. Ultrastructural studies showed that BnSP-7 caused severe morphological changes in promastigotes such as mitochondrial swelling, nuclear alteration, vacuolization, acidocalcisomes, multiflagellar aspects and a blebbing effect in the plasma membrane. Finally, BnSP-7 interfered with the infective capacity of promastigotes in murine peritoneal macrophages, causing statistically significant infectivity-index reductions (P < 0·05) of 20–35%. These data suggest that the BnSP-7 toxin is an important tool for the discovery of new parasite targets that can be exploited to develop new drugs for treating leishmaniasis.

Keywords

basic phospholipase A2Bothrops pauloensiscytotoxicityLeishmania (Leishmania) amazonensis
Type
Research Article
Information
Parasitology , Volume 140 , Issue 7 , June 2013 , pp. 844 - 854
Copyright
Copyright © Cambridge University Press 2013 

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