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What has proteomics taught us about Leishmania development?

Published online by Cambridge University Press:  28 February 2012

POLINA TSIGANKOV ,
PIER FEDERICO GHERARDINI ,
MANUELA HELMER-CITTERICH  and
DAN ZILBERSTEIN
POLINA TSIGANKOV
Affiliation:
Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
PIER FEDERICO GHERARDINI
Affiliation:
Centre for Molecular Bioinformatics, Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, Rome, Italy
MANUELA HELMER-CITTERICH
Affiliation:
Centre for Molecular Bioinformatics, Department of Biology, University of Rome Tor Vergata, Via della Ricerca Scientifica, Rome, Italy
DAN ZILBERSTEIN*
Affiliation:
Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel
*
*Corresponding author: Dan Zilberstein, Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel. Tel: 972-4-8293647; Fax: 972-4-8225153; E-mail: danz@tx.technion.ac.il
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Summary

Leishmania are obligatory intracellular parasitic protozoa that cycle between sand fly mid-gut and phagolysosomes of mammalian macrophages. They have developed genetically programmed changes in gene and protein expression that enable rapid optimization of cell function according to vector and host environments. During the last two decades, host-free systems that mimic intra-lysosomal environments have been devised in which promastigotes differentiate into amastigotes axenically. These cultures have facilitated detailed investigation of the molecular mechanisms underlying Leishmania development inside its host. Axenic promastigotes and amastigotes have been subjected to transcriptome and proteomic analyses. Development had appeared somewhat variable but was revealed by proteomics to be strictly coordinated and regulated. Here we summarize the current understanding of Leishmania promastigote to amastigote differentiation, highlighting the data generated by proteomics.

Keywords

Leishmaniaintracellular parasitismproteomicsdifferentiation
Type
Research Article
Information
Parasitology , Volume 139 , Issue 9: Symposia of the British Society for Parasitology Volume 48 Proteomic insights into parasite biology , August 2012 , pp. 1146 - 1157
Copyright
Copyright © Cambridge University Press 2012

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