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Changes in protein expression in the sheep abomasum following trickle infection with Teladorsagia circumcincta

Published online by Cambridge University Press:  11 November 2011

ALAN D. PEMBERTON ,
JEREMY K. BROWN ,
NICKY M. CRAIG ,
JUDITH PATE ,
KEVIN McLEAN ,
NEIL F. INGLIS ,
DAVID KNOX  and
PAMELA A. KNIGHT
ALAN D. PEMBERTON*
Affiliation:
The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, UK
JEREMY K. BROWN
Affiliation:
The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, UK
NICKY M. CRAIG
Affiliation:
The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, UK
JUDITH PATE
Affiliation:
The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, UK
KEVIN McLEAN
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK
NEIL F. INGLIS
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK
DAVID KNOX
Affiliation:
Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK
PAMELA A. KNIGHT
Affiliation:
The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, UK
*
*Corresponding author: The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian EH25 9RG, UK. E-mail: alan.pemberton@ed.ac.uk
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Summary

Continual low-level exposure of sheep to the helminth Teladorsagia circumcincta elicits a temporary protective immunity, where factors in the immune abomasal mucosa prevent penetration of infective larvae, but which is essentially lost within 6 weeks of cessation of parasite challenge. Here, a proteomic approach was used to identify proteins that are differentially regulated in immune compared to naïve sheep, as potential key mediators of immunity. Six naïve sheep and 12 sheep trickle-infected with T. circumcincta were treated with anthelmintic, and the naïve (control) and 6 immune sheep were killed 7 days later. The remaining 6 sheep (immune waning) were killed 42 days after anthelmintic treatment. Abomasal tissue samples were subjected to 2D-gel electrophoresis and densitometric analysis. Selected spots (n=73) were identified by peptide mass fingerprinting and confirmatory Western blotting was carried out for 10 proteins. Spots selectively up-regulated in immune versus control, but not immune waning versus control sheep, included galectin-15 and thioredoxin, which were confirmed by Western blotting. In immune sheep, serum albumin was significantly down-regulated and albumin proteolytic cleavage fragments were increased compared to controls. Unexpectedly, albumin mRNA was relatively highly expressed in control mucosa, down-regulated in immune, and was immunolocalized to mucus-producing epithelial cells. Thus we have identified differential expression of a number of proteins following T. circumcincta trickle infection that may play a role in host protection and inhibition of parasite establishment.

Keywords

sheepTeladorsagia circumcinctaabomasumalbumingalectin-15intelectin
Type
Research Article
Information
Parasitology , Volume 139 , Issue 3 , March 2012 , pp. 375 - 385
Copyright
Copyright © Cambridge University Press 2011

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Pemberton Supplementary Table 1

Supplementary Table 1: Sheep abomasal proteins identified by peptide mass fingerprinting

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