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Proteomic analysis of a 120 kDa protein complex in cyst fluid of Taenia solium metacestode and preliminary evaluation of its value for the serodiagnosis of neurocysticercosis

Published online by Cambridge University Press:  15 August 2005

E.-G. LEE ,
Y.-A. BAE ,
Y.-T. JEONG ,
J.-Y. CHUNG ,
E.-Y. JE ,
S.-H. KIM ,
B.-K. NA ,
J.-W. JU ,
T.-S. KIM  and
L. MA
...Show all authors
E.-G. LEE
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
Y.-A. BAE
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
Y.-T. JEONG
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
J.-Y. CHUNG
Affiliation:
PharmacoGenomics Research Center, Inje University, Busan 614-735, Korea
E.-Y. JE
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
S.-H. KIM
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
B.-K. NA
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
J.-W. JU
Affiliation:
Department of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
T.-S. KIM
Affiliation:
Department of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
L. MA
Affiliation:
Infectious Diseases Section, Department of Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
S.-Y. CHO
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
Y. KONG
Affiliation:
Department of Molecular Parasitology and Center for Molecular Medicine, Samsung Biomedical Research Institute and Sungkyunkwan University School of Medicine, Suwon 440-746, Korea
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Abstract

Cyst fluid (CF) of Taenia solium metacestode (TsM) is an important source of serodiagnostic antigens. We have investigated the molecular characteristics of the 120 kDa protein complex in TsM CF purified by fast performance liquid chromatography. The structure of the purified protein was characterized by a variety of proteomic analyses. The protein was found to consist of 2 major components of 42–46 and 22–28 kDa, and shared 3 subunits of 14, 16 and 18 kDa. The 42–46 kDa component was determined to contain 3 additional subunits of 22, 28 and 38 kDa. These 6 subunits were shown to originate from either the 14 or 18 kDa precursor. We assessed the antibody reactivity of the native protein, its individual subunits and the recombinant 14 and 18 kDa proteins, and found that the 120 kDa protein, particularly 14 and 18 kDa subunits revealed high reliability for differentiation of active and mixed stage NC from chronic NC. The subunits of the 120 kDa protein complex identified herein represent some of the low-molecular weight glycoproteins which have been described in several previous studies. Recognizing and understanding the structural and immunological relationship of these proteins will facilitate the development of new serodiagnostic assays.

Keywords

Taenia solium neurocysticercosis120 kDa glycoproteinMALDI-TOF MS14 kDa18 kDalow-molecular weight glycoproteinserodiagnostic antigen
Type
Research Article
Information
Parasitology , Volume 131 , Issue 6 , December 2005 , pp. 867 - 879
Copyright
© 2005 Cambridge University Press

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