Hostname: page-component-8448b6f56d-jr42d Total loading time: 0 Render date: 2024-04-20T09:10:07.949Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of a low level primary Schistosoma japonicum infection on establishment of a challenge infection in pigs

Published online by Cambridge University Press:  09 October 2003

I. T. PEDERSEN ,
M. V. JOHANSEN  and
N. ØRNBJERG
I. T. PEDERSEN
Affiliation:
Danish Bilharziasis Laboratory, Jaegersborg Allé 1D, DK-2920 Charlottenlund, Denmark Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Ridebanevej 3, DK-1870 Frederiksberg C, Denmark
M. V. JOHANSEN
Affiliation:
Danish Bilharziasis Laboratory, Jaegersborg Allé 1D, DK-2920 Charlottenlund, Denmark Danish Centre for Experimental Parasitology, Royal Veterinary and Agricultural University, Ridebanevej 3, DK-1870 Frederiksberg C, Denmark
N. ØRNBJERG
Affiliation:
Danish Bilharziasis Laboratory, Jaegersborg Allé 1D, DK-2920 Charlottenlund, Denmark
Get access Rights & Permissions [Opens in a new window]

Abstract

The aim of the study was to investigate the effect of a low-level primary infection of Schistosoma japonicum on a challenge infection in pigs. Groups of pigs were given either light or heavy primary infections and heavy challenge infections 14 weeks later. One group of pigs served as a challenge control group. Challenge infection superimposed on the heavily infected pigs did not result in increased worm burden, faecal and tissue egg counts or IgG levels. In contrast, the challenge infection established fully when superimposed on the light primary infections. However, neither faecal egg counts nor the IgG levels increased, and the amount of liver pathology, as judged by septal fibrosis, did not increase either, as compared to the challenge control group. These results suggest that pigs are not able to mount an effective anti-worm response to reinfection when the primary infection is low. However, some modulation of the infection takes place, possibly as a result of an anti-embryonation effect. Collagen content in the liver was found to be relatively insensitive as a marker for liver pathology, as judged using portal and septal fibrosis as a criteria.

Keywords

Schistosoma japonicumpigsresistance
Type
Research Article
Information
Parasitology , Volume 127 , Issue 2 , August 2003 , pp. 121 - 130
Copyright
2003 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

BØGH, H. O., WILLINGHAM, A. L., BARNES, E. H., JOHANSEN, M. V., CHRISTENSEN, N. Ø. & NANSEN, P. (1996). A methodological study on egg counts in the tissues from pigs infected with Schistosoma japonicum. Veterinary Parasitology 65, 21–27.CrossRefGoogle Scholar
BØGH, H. O., WILLINGHAM, A. L., JOHANSEN, M. V., ERIKSEN, L. & CHRISTENSEN, N. Ø. (1997). Recovery of Schistosoma japonicum from experimentally infected pigs by perfusion of liver and mesenteric veins. Acta Veterinaria Scandinavica 38, 147–156.Google Scholar
ETHERINGTON, D. J. & SIMS, T. J. (1981). Detection and estimation of collagen. Journal of Science and Food Agriculture 32, 539–546.CrossRefGoogle Scholar
GIVER, H., JOHANSEN, M. V., CHRISTENSEN, N. Ø. & NANSEN, P. (1999). Schistosoma japonicum in the pig: A new technique for estimation of intestinal tissue egg counts. Southeast Asian Journal of Tropical Medicine and Public Health 30, 664–669.Google Scholar
GIVER, H., DE VLAS, S. J., JOHANSEN, M. V., CHRISTENSEN, N. Ø. & NANSEN, P. (2000). Schistosoma japonicum: day to day variation in excretion and hatchability of parasite eggs in the domestic pig, Suis suis. Experimental Parasitology 95, 8–18.CrossRefGoogle Scholar
HURST, M. H., WILLINGHAM, A. L. & LINDBERG, R. (2000). Tissue responses in experimental schistosomiasis japonica in the pig: a histopathologic study of different stages of single low- or high dose infections. American Journal of Tropical Medicine and Hygiene 62, 45–56.CrossRefGoogle Scholar
JOHANSEN, M. V., FILLIE, Y., MONRAD, J., CHRISTENSEN, N. Ø. & DEELDER, A. (1996). Experimental Schistosoma bovis in goats. Circulating antigen and antibody responses to egg and adult worm antigens during infection and following treatment with praziquantel. Parasitology 113, 367–375.Google Scholar
JOHANSEN, M. V., BØGH, H. O., NANSEN, P. & CHRISTENSEN, N. Ø. (2000). Schistosoma japonicum infection in the pig as a model for human schistosomiasis japonica. Acta Tropica 76, 85–99.CrossRefGoogle Scholar
JOHANSEN, M. V., IBURG, T., BØGH, H. O. & CHRISTENSEN, N. Ø. (2001). Postnatal challenge infections of congenitally Schistosoma japonicum-infected piglets. Journal of Parasitology 87, 813–815.Google Scholar
KARDORFF, R., ERIKSEN, L., NIELSEN, D. H. & JOHANSEN, M. V. (2003). Validation of ultrasonography for hepatic schistosomiasis using a porcine Schistosoma japonicum model. Acta Tropica 85, 315–323.CrossRefGoogle Scholar
LI, Y. S., ROSS, A. G. P., SLEIGH, A. C., LI, Y., WAINE, G. J., WILLIAMS, G. J., TANNER, M. & McMANUS, D. P. (1999). Antibody isotype responses, infection for Schistosoma japonicum in a marshland area of China. Acta Tropica 73, 79–92.CrossRefGoogle Scholar
MITCHELL, G. F., GARCIA, E. G., RIVERA, P. T., TIU, W. U. & DAVERN, K. M. (1994). Minireview: Evidence for and implications of anti-embryonation immunity in schistosomiasis. Experimental Parasitology 79, 546–549.CrossRefGoogle Scholar
MURRELL, K. D., YOGORE, M. G., LEWERT, R. M., CLUTTER, W. G. & VANNIER, W. E. (1973). Immunization with a zoophilic strain of Schistosoma japonicum: a re-evaluation of the Formosan strain of S. japonicum in Rhesus monkeys. The American Journal of Tropical Medicine and Hygiene 22, 723–733.CrossRefGoogle Scholar
NESSIM, G. N. & DEMERDASH, C. (2000). Correlation between infection intensity, serum immunoglobulin profile, cellular immunity and the efficacy of treatment with praziqantel in murine schistosomiasis mansoni. Arzneimittel – Forchung/Drug Research 50, 173–177.Google Scholar
SØRENSEN, E., JOHANSEN, M. V., WILSON, S. & BØGH, H. O. (1999). Elucidation of Schistosoma japonicum population dynamics in pigs using PCR-based identification of individuals representing distinct cohorts. International Journal for Parasitology 29, 1907–1915.CrossRefGoogle Scholar
PHILLIPS, R. V. & TUMBLESON, M. E. (1986). Models. In Swine in Biomedical Research(ed. Tumbleson, M. E.), pp. 437–440. Plenum Press, New York.
WILLINGHAM, A. L., BØGH, H. O., JOHANSEN, M. V., CHRISTENSEN, N. Ø. & NANSEN, P. (1997). Schistosoma japonicum infection in the pig: the effect of a patent primary infection on a challenge infection. ActaTropica 66, 51–59.CrossRefGoogle Scholar
WILLINGHAM, A. L., BØGH, H. O., VENNERVALD, B. J., JOHANSEN, M. V., ERIKSEN, L., CHRISTENSEN, N. Ø. & NANSEN, P. (1996). Worm establishment and egg production of Schistosoma japonicum in pigs infected by percutaneous methods or intramuscular injection. Veterinary Parasitology 61, 157–163.CrossRefGoogle Scholar
WILLINGHAM, A. L. & HURST, M. (1996). The pig as a unique host model for Schistosoma japonicum infection. Parasitology Today 12, 132–134.CrossRefGoogle Scholar
WILLINGHAM, A. L., HURST, M., BØGH, H. O., JOHANSEN, M. V., LINDBERG, R., CHRISTENSEN, N. Ø. & NANSEN, P. (1998). Schistosoma japonicum in the pig: The host-parasite relationship as influenced by the intensity and duration of experimental infection. American Journal of Tropical Medicine and Hygiene 58, 248–256.CrossRefGoogle Scholar
WILLINGHAM, A. L., JOHANSEN, M. V. & BARNES, E. H. (1998). A new technic for counting Schistosoma japonicum eggs in pig feces. Southeast Asian Journal of Tropical Medicine and Public Health 29, 128–130.Google Scholar
WILLINGHAM, A. L., JOHANSEN, M. V., BØGH, H. O., ITO, A., ANDREASEN, J., LINDBERG, R., CHRISTENSEN, N. Ø. & NANSEN, P. (1999). Congenital transmission of Schistosoma japonicum in pigs. American Journal of Tropical Medicine and Hygiene 60, 311–312.CrossRefGoogle Scholar
WYLER, D. J. (1992). Why does liver fibrosis occur in schistosomiasis. Parasitology Today 8, 277–279.CrossRefGoogle Scholar
WYLER, O. D. (1972). Routine-Untersuchungsmethoden für Fleisch und Fleischwaren. Die Fleischwirtschaft 1, 42–44.Google Scholar
YASON, C. V. & NOVILLA, M. N. (1984). Clinical and pathological features of experimental Schistosoma japonicum infection in pigs. Veterinary Parasitology 17, 47–64.CrossRefGoogle Scholar