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A phylogeny based on three mitochondrial genes supports the division of Schistosoma intercalatum into two separate species

Published online by Cambridge University Press:  09 October 2003

R. A. KANE ,
V. R. SOUTHGATE ,
D. ROLLINSON ,
D. T. J. LITTLEWOOD ,
A. E. LOCKYER ,
J. R. PAGÈS ,
L. A. TCHUEM TCHUENTÉ  and
J. JOURDANE
R. A. KANE
Affiliation:
Biomedical Parasitology Division, Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
V. R. SOUTHGATE
Affiliation:
Biomedical Parasitology Division, Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
D. ROLLINSON
Affiliation:
Biomedical Parasitology Division, Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
D. T. J. LITTLEWOOD
Affiliation:
Parasitic Worms Division, Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
A. E. LOCKYER
Affiliation:
Parasitic Worms Division, Department of Zoology, The Natural History Museum, Cromwell Road, London SW7 5BD
J. R. PAGÈS
Affiliation:
Centre de Biologie et d'Ecologie Tropicale et Méditerranéenne (UMR 5555 du CNRS), Université de Perpignan, Av. De Villeneuve, 66860 Perpignan Cedex, France
L. A. TCHUEM TCHUENTÉ
Affiliation:
Laboratoire de Biologie Générale, Faculté des Sciences, Université de Yaoundé I, B.P. 812 Yaoundé, Cameroun
J. JOURDANE
Affiliation:
Centre de Biologie et d'Ecologie Tropicale et Méditerranéenne (UMR 5555 du CNRS), Université de Perpignan, Av. De Villeneuve, 66860 Perpignan Cedex, France
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Abstract

Two recognized strains of Schistosoma intercalatum, one from the Democratic Republic of Congo (DRC), formerly Zaire, and the other from Cameroon, have been investigated using DNA sequences from 3 mitochondrial genes, cytochrome oxidase subunit 1 (cox1), NADH dehydrogenase subunit 6 (nad6) and the small ribosomal RNA gene (rrnS). In addition, partial DNA sequences from the nuclear large subunit ribosomal RNA gene (lsrDNA) were included within the study. Although partial lsrDNA alone reveals little taxonomic information, phylogenetic analysis of the mitochondrial data demonstrates a clear dichotomy between the 2 purported strains and it is proposed that they should be treated as distinct taxa. The ‘original’ S. intercalatum now falls relatively basal in the S. haematobium group, while the proposed new species is more derived and sister taxon to S. bovis and S. curassoni.

Keywords

Schistosoma intercalatummolecular diversityphylogenycox1nad6rrnSlsrDNA
Type
Research Article
Information
Parasitology , Volume 127 , Issue 2 , August 2003 , pp. 131 - 137
Copyright
2003 Cambridge University Press

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