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The formation and turnover of the membranocalyx on the tegument of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

R. A. Wilson
Affiliation:
Department of Biology, University of York, Heslington, York
P. E. Barnes
Affiliation:
Department of Biology, University of York, Heslington, York

Extract

The multilaminate vesicles present in the tegument cytoplasm appear to fuse with side channels projecting out into the cytoplasm from the base of the surface pits. Their lamellate contents then unroll and spread out to form a trilaminate membranocalyx lining the pits and covering the tegument surface. The plant lectin concanavalin A appears to stabilize the process of vesicle fusion leading to an aggregation of multilaminate vesicles trapped in the lumen of the surface pits. The membranocalyx can be labelled with cationized ferritin. Chase incubations in normal medium indicate that by 4 h most of the label and the membranocalyx to which it is bound have been lost to the medium. This suggests that under the conditions of these experiments the membranocalyx has a half-life of 2–3 h.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1977

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References

Amsterdam, A., Ohad, I. & Schramm, M. (1969). Dynamic changes in the ultrastructure of the acinar cell of the rat parotid gland during the secretory cycle. Journal of Cell Biology 41, 753–73.CrossRefGoogle ScholarPubMed
Capron, A., Biguet, J., Vernes, A. & Afchain, D. (1968). Structure antigénique des helminthes. Aspects immunologiques des relations hôte–parasite. Pathologie-Biologie 16, 121–38.Google Scholar
Chowdhury, T. K. & Weiss, A. K. (1975). Concanavalin A. In Advances in Experimental Medicine and Biology Vol. 55. New York and London: Plenum Press.Google Scholar
Clegg, J. A. (1972). The schistosome surface in relation to parasitism. Functional Aspects of Parasite Surfaces: Symposium of the British Society for Parasitology, Vol. 10 (ed. Taylor, A. E. R. and Muller, R.), pp. 2340. Oxford and London: Blackwell.Google Scholar
Damian, R. T., Greene, N. D. & Hubbard, W. J. (1973). Occurrence of mouse α2-macro-globulin antigenic determinants on Schistosoma mansoni adults, with evidence on their nature. Journal of Parasitology 59, 6473.CrossRefGoogle Scholar
Erasmus, D. A. (1967). The host-parasite interface of Cyathocotyle bushiensis Khan, 1962 (Trematoda: Strigeoidea). II. Electron microscope studies of the tegument. Journal of Parasitology 53, 703–14.CrossRefGoogle ScholarPubMed
Frye, J. D. & Edidin, E. (1970). The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. Journal of Cell Science 7, 319–35.CrossRefGoogle ScholarPubMed
Hereward, F. V. & Northcote, D. H. (1972). A simple freeze-substitution method for the study of ultrastructure of plant tissues. Experimental Cell Research 70, 7380.CrossRefGoogle Scholar
Hockley, D. J. & McLaren, D. J. (1973). Schistosoma mansoni: changes in the outer membrane of the tegument during development from cercaria to adult worm. International Journal for Parasitology 3, 1325.CrossRefGoogle ScholarPubMed
Hockley, D. J., McLaren, D. J., Ward, B. H. & Nermut, M. V. (1975). A freeze-fracture study of the tegumental membrane of Schistosoma mansoni (Platyhelminthes: Trematoda). Tissue and Cell 7, 485–96.CrossRefGoogle ScholarPubMed
Jamieson, J. D. (1972). Transport and discharge of exportable proteins in pancreatic exocrine cells: in vitro studies. In Current Topics in Membranes and Transport, Vol. 3 (ed. Bronner, F. and Kleinzeller, A.), pp. 273338. New York and London: Academic Press.Google Scholar
Karnovsky, M. J. (1965). A formaldehyde–glutaraldehyde fixative of high osmolarity for use in election microscopy. Journal of Cell Biology 27, 137A.Google Scholar
Kusel, J. R. & Mackenzie, P. E. (1975). The measurement of relative turnover rates of proteins of the surface membranes and other fractions of Schistosoma mansoni in culture. Parasitology 71, 261–73.CrossRefGoogle ScholarPubMed
Kusel, J. R., Mackenzie, P. E. & McLaren, D. J. (1975). The release of membrane antigens into culture medium by adult Schistosoma mansoni. Parasitology 71, 247–59.CrossRefGoogle ScholarPubMed
Mollenhauer, H. H. (1969). In Principles and Techniques of Electron Microscopy, Vol. 1 (ed. Hayat, M. A.), p. 341. New York: Van Nostrand Reinhold.Google Scholar
Palade, G. (1975). Intracellular aspects of the process of protein synthesis. Science 189, 347–58.CrossRefGoogle ScholarPubMed
Perez, H. & Terry, R. J. (1973). The killing of adult Schistosoma mansoni in vitro in the presence of antisera to host antigenic determinants and peritoneal cells. International Journal for Parasitology 3, 499503.CrossRefGoogle ScholarPubMed
Robards, A. W. (1974). Ultrastructural methods for looking at frozen cells. Science Progress 61, 140.Google ScholarPubMed
Smith, S. B. & Revel, J. P. (1972). Mapping of concanavalin A binding sites on the surface of several cell types. Developmental Biology 27, 434–41.CrossRefGoogle ScholarPubMed
Warren, L. & Glick, M. C. (1968). Membranes of animal cells. II. The metabolism and trunover of the surface membrane. Journal of Cell Biology 37, 729–46.CrossRefGoogle Scholar
Wheater, P. R. & Wilson, R. A. (1976). The tegument of Schistosoma mansoni: a histochemical investigation. Parasitology 72, 99109.CrossRefGoogle ScholarPubMed
Wilson, R. A. (1972). Gland cells in the redia of Fasciola hepatica. Parasitology 65, 433–6.CrossRefGoogle ScholarPubMed
Wilson, R. A. & Barnes, P. E. (1974 a). The tegument of Schistosoma mansoni: observations on the formation, structure and composition of cytoplasmic inclusions in relation to tegument function. Parasitology 68, 239–58.CrossRefGoogle ScholarPubMed
Wilson, R. A. & Barnes, P. E. (1974 b). An in vitro investigation of dynamic processes occurring in the schistosome tegument, using compounds known to disrupt secretory processes. Parasitology 68, 259–70.CrossRefGoogle Scholar
Zalokar, M. (1966). A simple freeze-substitution method for electron microscopy. Journal of Ultrastructure Research 15, 469–79.CrossRefGoogle ScholarPubMed