Hostname: page-component-8448b6f56d-sxzjt Total loading time: 0 Render date: 2024-04-16T05:10:33.077Z Has data issue: false hasContentIssue false
  • English
  • Français

First assessment of Perkinsosis and brown ring disease co-infection in Ruditapes decussatus in the North Lake of Tunis (southern Mediterranean Sea)

Published online by Cambridge University Press:  09 December 2012

Monia El Bour ,
Mohamed Dellali ,
Imene Boukef ,
Fatma Lakhal ,
Radhia Mraouna ,
Hedia Attia El Hili ,
Christine Paillard  and
John Klena
Monia El Bour*
Affiliation:
Laboratoire de Pathologie des Organismes Aquatiques, Institut National Des Sciences et Technologies de la Mer (INSTM), Rue du 2 mars 1934, 2025, Salammbô, Tunisie
Mohamed Dellali
Affiliation:
Laboratoire d'Ecologie et de Bio-surveillance Côtière, FSB, 7021, Zarzouna, Tunisie
Imene Boukef
Affiliation:
Laboratoire de Pathologie des Organismes Aquatiques, Institut National Des Sciences et Technologies de la Mer (INSTM), Rue du 2 mars 1934, 2025, Salammbô, Tunisie
Fatma Lakhal
Affiliation:
Laboratoire de Pathologie des Organismes Aquatiques, Institut National Des Sciences et Technologies de la Mer (INSTM), Rue du 2 mars 1934, 2025, Salammbô, Tunisie
Radhia Mraouna
Affiliation:
Laboratoire de Pathologie des Organismes Aquatiques, Institut National Des Sciences et Technologies de la Mer (INSTM), Rue du 2 mars 1934, 2025, Salammbô, Tunisie
Hedia Attia El Hili
Affiliation:
Laboratoire de Pathologie des Organismes Aquatiques, Institut National Des Sciences et Technologies de la Mer (INSTM), Rue du 2 mars 1934, 2025, Salammbô, Tunisie
Christine Paillard
Affiliation:
Institut Européen de la Mer, Laboratoire des Sciences de l'Environnement Marin—LEMAR,UMR 6539, Université de Bretagne Occidentale (CNRS), Technopôle Brest–Iroise, Place Nicolas Copernic, 29280 Plouzané, France
John Klena
Affiliation:
United States Centers for Disease Control and Prevention, Unit 7300, Box 0066, USA
*
Correspondence should be addressed to: M.E. Bour, Laboratoire de Pathologie des Organismes Aquatiques, Institut National Des Sciences et Technologies de la Mer (INSTM), Rue du 2 mars 1934, 2025, Salammbô, Tunisie email: monia.elbour@instm.rnrt.tn
Get access Rights & Permissions [Opens in a new window]

Abstract

Carpet shell clam populations on the Tunisian coastline are susceptible to several microbial pathogen challenges. In this study we report the results of five years' surveillance, conducted from January 2004 till June 2009, for detection of Perkinsosis and brown ring disease (BRD). The survey covered three sites of natural populations of Ruditapes decussatus in a Tunisian lagoon, the North Lake of Tunis. Perkinsosis was detected preferentially in winter periods from the external and marine site, BRD was detected more frequently in the summer periods in a more proximal collection site (in the lake) and was positively correlated with concentrations of heterotrophic Vibrio sp. Our results suggest that several factors other than temperature and salinity might explain spatial distribution variability and natural intensities for these infections in carpet shell clam populations.

Keywords

Ruditapes decussatusPerkinsosisbrown ring diseaseprevalenceenvironmental factors
Type
Research Article
Information
Journal of the Marine Biological Association of the United Kingdom , Volume 92 , Issue 7 , November 2012 , pp. 1579 - 1584
Copyright
Copyright © Marine Biological Association of the United Kingdom 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Allam, B., Paillard, C., Auffret, M. and Ford, S.E. (2006) Effect of the pathogenic Vibrio tapetis on defence factors of susceptible and non-susceptible bivalve species: II. Cellular and biochemical changes following in vivo challenge. Fish and Shellfish Immunology 20, 384–397.Google Scholar
Armi, Z., Turki, S., Trabelsi, E. and Ben Maiz, N. (2009) Nutrient loading and occurrence of potentially harmful phytoplankton species in North Lake of Tunis (Tunisia). Cahiers de Biologie Marine 49, 311–321.Google Scholar
Belkhir, M., Gharbi, H., Dridi, K., Amara, H., Ksouri, A. and Hadj Ali Salem, M. (1985) Le lac de Bizerte: étude physico-chimique et microbiologique de l'écosystème lagunaire. Rapports et Documents 3, 1–22 Google Scholar
Ben Charrada, R. (1992) Le lac de Tunis après les aménagements: paramètres physico-chimiques de l'eau et relation avec la croissance des algues. Marine Life 1, 29–44.Google Scholar
Ben Salah, C. (2005) Etude parasitaire et lésionnelle chez la palourde (R. decussatus) dans le lac de Bizerte. Thèse de Médecine Vétérinaire. Université de Tunis El Manar, 58 pp.Google Scholar
Borrego, J.J., Luque, A., Castro, D., Santamaria, J.A. and Martinez-Manzares, E. (1996) Virulence factors of the Vibrio PI, the causative agent of brown ring disease in the Manila clam, Ruditapes philippinarum : Aquatic Living Resources 9, 125–136.Google Scholar
Castro, D., Pujalte, M.J., Lopez-Cortes, L., Garay, E. and Borrego, J.J. (2002) Vibrios isolated from the cultured Manila clam (R. philippinarum): numerical taxonomy and antibacterial activities. Journal of Applied Microbiology 93, 438–447.Google Scholar
Ciacci, C., Citterio, B., Betti, M., Canonico, B., Roch, P. and Canesi, L. (2009) Functional differential immune responses of M. galloprovincialis to bacterial challenge. Comparative Biochemistry and Physiology. Series B. Biochemistry and Molecular Biology 153, 365–371.Google Scholar
Da Dang, C. and Montadouin, X. (2009) Brown muscle disease and Manila clam Ruditapes philipinarum dynamics in Arcachon Bay, France. Journal of Shellfish Research 28, 355–362.CrossRefGoogle Scholar
El Bour, M., Jaafar-kéfi, F., Hamrouni, M., Mahmoud, N., Dellali, M., Trigui-El-Menif, N. and Aloui-Bejaoui, N. (2008) Etude de dépistage de la maladie de l'anneau brun chez des espèces de bivalves des côtes tunisiennes. Bulletin de la Société Zoologique de France 133 107–115.Google Scholar
El Hili, H., Ben Salah, C., Ayari, W. and Ben Amor, N. (2007) Etude descriptive de l'infestation de la palourde (R. decussatus) à Perkinsus olseni dans le lac de Bizerte en Tunisie. Rapport de la Commission International de la Mar Méditerranée 38, 421.Google Scholar
Flye-Sainte-Marie, J., Pouvreau, S., Paillard, C. and Jean, F. (2007) Impact of brown ring disease on the energy budget of the Manila clams Ruditapes philippinarum . Journal of Experimental Marine Biology and Ecology 349 378–389.Google Scholar
Flye-Sainte-Marie, J., Jean, F., Ford, S.E. and Paillard, C. (2008) Effect of grain-size on development of brown ring disease in Manila clam Ruditapes philippinarum . Aquaculture 278 184–187.Google Scholar
Fly-Sainte-Marie, J., Soudant, P., Lambert, C., Le Goïc, N., Goncalvez, M., Travers, A. and Paillard, C. (2009) Variability of the hemocyte parameters of R. philippinarum in the field during an annual cycle. Journal of Experimental Marine Biology and Ecology 377, 1–11.Google Scholar
Labreuche, Y., Soudant, P., Goncalves, M., Lambert, C. and Nicolas, J.L. (2006) Effect of extra-cellular products from pathogenic Vibrio aestuarinus strain O1/O32 on lethality and cellular immune responses of the oyster Crassostrea gigas . Developmental and Comparative Immunology 30, 367–379.CrossRefGoogle Scholar
Lassalle, G., Montaudouin, X., Soudant, P. and Paillard, C. (2007) Parasite co-infection of two sympatric bivalves, the Manila clam (R. philippinarum) and the cockle (Cerastoderma edule) along a latitudinal gradient. Aquatic Living Resources 20, 33–42.Google Scholar
Leite, R.B., Alfonso, R. and Cancela, M.L. (2004) Perkinsus sp infestation in carpet-shell clam (R. decussatus), along the Portuguese coast. Results from a 2-year survey. Aquaculture 240, 39–53.Google Scholar
Ngo, T.T.T and Choi, K.S. (2004) Seasonal changes of Perkinsus and Cercaria infections in the Manila clam R. philippinarum from Jeju, Korea. Aquaculture 239, 57–68.CrossRefGoogle Scholar
Nolwenn-Trinkler, T., Sinquin, G., Querne, J. and Paillard, C. (2010) Resistance to brown ring disease in the Manila clam Ruditapes philippinarum: a study of selected stocks showing a recovery process by shell repair. Journal of Invertebrate Pathology 104, 8–16.Google Scholar
OIE (2001) Diagnostic manual for Aquatic diseases. 3rd edition. Paris: Office International des Epizooties, 273 pp.Google Scholar
Paillard, C., Maes, P. and Oubella, R. (1994) Brown ring disease in clams. Annual Review of Fish Diseases 4, 219–240.Google Scholar
Paillard, C., Maes, P. and Oubella, R. (2004) Effect of temperature on defence parameters in Manila clams Ruditapes philippinarum challenged with Vibrio tapetis . Diseases of Aquatic Organisms 59, 249–262.Google Scholar
Paillard, C., Gausson, S., Nicolas, J.L., Le Pennec, J.P. and Haras, D. (2006) Molecular identification of Vibrio tapetis, the causative agent of the brown ring disease of Ruditapes philippinarum . Aquaculture, 253, 25–38.Google Scholar
Park, K.I. and Choi, K.S. (2001) Spatial distribution of the protozoom parasite Perkinus sp. found in the Manila clams, Ruditapes philippinarum, in Korea. Aquaculture 203, 9–22.CrossRefGoogle Scholar
Park, K.L., Paillard, C., Le Chevalier, P. and Choi, K.S. (2006a) Report on the occurrence of brown ring disease (BRD) in Manila clam, Ruditapes philippinarum, on the west coast of Korea. Aquaculture 255, 610–613.CrossRefGoogle Scholar
Park, K.I. Figuers, A. and Choi, K.S. (2006b) Application of enzyme-linked immunosorbent assay (ELISA) for the study of reproduction in the Manila clam Ruditapes philippinarum (Mollusca: Bivalvia). II. Impacts of Perkinsus olseni on clam reproduction. Aquaculture 251, 182–191.Google Scholar
Samain, J.F., Dégremont, L., Soletchnik, P., Hauré, J., Bédier, E., Ropert, M., Moal, J., Huvet, A., Bacca, H., Van Wormhoudt, A., Délaporte, M., Costil, K., Pouvreau, S., Lambert, C., Boulo, V., Soudant, P., Nicolas, J.L., Le Roux, F., Renault, T., Gagnaire, B., Géret, F., Boutet, I. and Boudry, P. (2007) Genetically based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infection process. Aquaculture 268, 227–243.Google Scholar
Soudant, P., Leite, R., Chu, F.L.E., Villaba, A. and Cancela, L. (2008) Bivalves–Perkinsus sp. interactions. In Villaba, A. (ed) Workshop for the analysis of the impact of Perkinsosis to the European shellfish industry. Centro de Investigacións Mariñas, Consellería de Pesca e Asuntos Marítimos da Xunta de Galicia, Vilanova de Arousa, Spain and Centro Tecnológico del Mar—Fundación CETMAR, Vigo, Spain. 2008, pp. 77–109.Google Scholar
Tuntiwaranuruk, C., Chalermwat, K., Upathum, E.S., Kruatrachue, M. and Azevedo, C. (2004) Investigation of Nematopsis sp. oocysts in 7 species of bivalves from Chonburi Province, Gulf of Thailand. Diseases of Aquatic Organisms 58, 47–53.Google Scholar
Villalba, A., Reece, K.S., Ordas, M.C., Casas, S.M. and Figueras, A. (2004) Perkinsosis in molluscs: a review. Aquatic Living Resources 17, 411–432.Google Scholar
Villalba, A., Casas, S.M., Lopez, C. and Carballal, M.J. (2005) Study of perkinsosis in the carpet shell clam Tapes decussatus in Galicia (NW Spain). II. Temporal pattern of disease dynamics and association with clam mortality. Diseases of Aquatic Organisms 65, 257–267.Google Scholar