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Duplication of the ribosomal gene cluster in the marine polychaete Platynereis dumerilii correlates with ITS polymorphism

Published online by Cambridge University Press:  04 April 2007

Jerome H.L. Hui
Affiliation:
Department of Zoology, University of Oxford, Tinbergen Building, South Parks Road, Oxford, OX1 3PS, UK
Natalia Kortchagina
Affiliation:
Department of Zoology, University of Oxford, Tinbergen Building, South Parks Road, Oxford, OX1 3PS, UK
Detlev Arendt
Affiliation:
Developmental Unit, European Molecular Biological Laboratory (EMBL), Meyerhofstrasse 1, D-69117 Heidelberg, Germany
Guillaume Balavoine
Affiliation:
Centre de Genetique Moleculaire du CNRS, Avenue de la Terrasse, 91198 Gif-sur-Yvette, France
David E.K. Ferrier
Affiliation:
Department of Zoology, University of Oxford, Tinbergen Building, South Parks Road, Oxford, OX1 3PS, UK

Abstract

Platynereis dumerilii is an emerging model system for both comparative developmental biology and ecotoxicology. As such it would be extremely valuable to be able to map the location of genes and markers in its genome, in order to understand how the genome changes or has changed, either due to chemical and environmental insults or during evolution. Here we report the adaptation of the technique of chromosomal fluorescence in situ hybridization (FISH) to P. dumerilii for the localization of single-copy genes. Also the duplicated ribosomal gene cluster of this annelid is more reliably revealed with FISH than with the previous technique of silver staining. This duplicated ribosomal cluster correlates with polymorphisms in the internal transcribed spacer (ITS) regions, which has implications for the use of these regions in phylogenetic or population biology work.

Type
Research Article
Copyright
2007 Marine Biological Association of the United Kingdom

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