Hostname: page-component-8448b6f56d-t5pn6 Total loading time: 0 Render date: 2024-04-24T00:42:17.462Z Has data issue: false hasContentIssue false
  • English
  • Français

A most probable number method for estimating small numbers of campylobacters in water

Published online by Cambridge University Press:  25 March 2010

F. J. Bolton ,
P. M. Hinchliffe ,
D. Coates  and
L. Robertson
F. J. Bolton
Affiliation:
Public Health Laboratory, Preston Infirmary, Meadow Street, Preston, Lancashire PR1 6PS, U.K.
P. M. Hinchliffe
Affiliation:
Public Health Laboratory, Preston Infirmary, Meadow Street, Preston, Lancashire PR1 6PS, U.K.
D. Coates
Affiliation:
Public Health Laboratory, Preston Infirmary, Meadow Street, Preston, Lancashire PR1 6PS, U.K.
L. Robertson
Affiliation:
Public Health Laboratory, Preston Infirmary, Meadow Street, Preston, Lancashire PR1 6PS, U.K.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A most probable number (MPN) method capable of estimating as few as ten campylobacters per 100 ml of water is described. The method gave results close to those obtained by the viable count method of Miles, Misra & Irwin (1938) with graded suspensions of Campylobacter jejuni. The method was used to test raw water samples: counts were obtained ranging from 10 to 230 campylobacters per 100 ml for 11 of 49 coastal and estuary water samples, and from 10 to 36 campylobacters per 100 ml for 7 of 44 river samples. Campylobacters were isolated from an additional 24 of the ‘negative’ samples by testing 200 ml volumes by glass microfibre filtration and enrichment culture methods. The MPN method should prove to be a useful epidemiological tool particularly suited to the enumeration of campylobacters in particulate fluids.

Type
Research Article
Information
Journal of Hygiene , Volume 89 , Issue 2 , October 1982 , pp. 185 - 190
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Blaser, M., Powers, B. W., Cravens, J. & Wang, W. L. (1978). Campylobacter enteritis associated with canine infection. Lancet ii, 979–981.CrossRefGoogle Scholar
Bolton, F. J. & Robertson, L. (1982). A selective medium for isolating Campylobacter jejuni/coli. Journal of Clinical Pathology 35, 462–67.CrossRefGoogle ScholarPubMed
Brouwer, R., Mertens, M. J. A., Siem, T. H. & Katchaki, J. (1979). An explosive outbreak of Campylobacter enteritis in soldiers. Antonie van Leeuwenhoek 45, 517–519.CrossRefGoogle ScholarPubMed
Campbell, R. C. (1974). Statistics for Biologists, 2nd ed.Cambridge University Press.Google Scholar
Diem, K. (ed). (1962). Documenta Geigy: Scientific Tables, Manchester: Geigy Pharmaceutical.Google Scholar
George, H. A., Hoffman, P. S., Smibert, R. M. & Kreig, N. R. (1978). Improved media for growth and aerotolerance of Campylobacter fetus. Journal of Clinical Microbiology 8, 36–41.CrossRefGoogle ScholarPubMed
Her Majesty's Stationery Office. (1969). The bacteriological examination of water supplies.Google Scholar
Knill, M., Suckling, W. G. & Pearson, A. D. (1978). Environmental isolation of heat-tolerant Campylobacter in the Southampton area. Lancet ii, 1002–1003.CrossRefGoogle Scholar
Mentzing, L. O. (1981). Waterborne outbreaks of Campylobacter enteritis in central Sweden. Lancet ii, 352–354.CrossRefGoogle Scholar
Miles, A. A., Misra, S. S. & Irwin, J. O. (1938). The estimation of the bactericidal power of the blood. Journal of Hygiene 38, 732–749.Google ScholarPubMed
Pearson, A. D.Suckling, W. G., Ricciardi, I. D., Knill, M. & Ware, E. (1977). Campylobacter associated diarrhoea in Southampton. British Medical Journal ii, 955–956.CrossRefGoogle Scholar
Robinson, D. A. & Jones, D. M. (1981). Milk-borne Campylobacter infection. British Medical Journal 282, 1374–1376.CrossRefGoogle ScholarPubMed
Skirrow, M. B. & Benjamin, J. (1980). Differentiation of enteropathogenic Campylobacter. Journal of Clinical Pathology 33, 1122.Google ScholarPubMed
Svedhem, A. & Norkrans, G. (1980). Campylobacter jejuni enteritis transmitted from cat to man. Lancet i, 713–714.CrossRefGoogle Scholar
Vogt, R. L., Sours, H. E., Barrett, T., Feldman, R. A., Dickinson, B. S. & Witherell, L. (1982). Campylobacter enteritis associated with contaminated water. Annals of Internal Medicine 96, 292–296.CrossRefGoogle ScholarPubMed
Wilson, G. S. & Miles, A. A. (1975). Topley and Wilson's Principles of Bacterioloy, Virology and Immunity, 6th ed., vol. ii. p. 2651. Edward Arnold.Google Scholar