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The production of mammalian trematode infective stages by the snail Galba truncatula

Published online by Cambridge University Press:  27 November 2012

D. Rondelaud ,
R. Mouzet ,
P. Vignoles ,
G. Dreyfuss  and
J. Cabaret
D. Rondelaud
Affiliation:
INSERM U 1094, Faculties of Medicine and Pharmacy, 87025Limoges, France
R. Mouzet
Affiliation:
INSERM U 1094, Faculties of Medicine and Pharmacy, 87025Limoges, France
P. Vignoles
Affiliation:
INSERM U 1094, Faculties of Medicine and Pharmacy, 87025Limoges, France
G. Dreyfuss*
Affiliation:
INSERM U 1094, Faculties of Medicine and Pharmacy, 87025Limoges, France
J. Cabaret
Affiliation:
UMR 1282 Infectiology and Public Health, INRA, 37380Nouzilly, France UMR 1282 Infectiology and Public Health, University of Tours, 37000Tours, France
*
*Fax: 33-555-435863 E-mail: gilles.dreyfuss@unilim.fr
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Abstract

Several experiments on the breeding of trematode-infected Galba truncatula for obtaining and packaging Fasciola hepatica and Paramphistomum daubneyi metacercariae were carried out to determine the more convenient methods to use for commercial production of these infective stages. Compared to the breeding of infected snails in aquaterraria, the use of 14-cm Petri dishes allowed a greater prevalence of snail infection and a higher number of metacercariae. The production of these larvae was still 2.3–3.4 times greater if infected snails were dissected during the patent period. The aspiration of these metacercariae at the extremity of a Pasteur pipette significantly shortens the time necessary for their transfer from Petri dishes to Eppendorf tubes. Using 14-cm Petri dishes, snail dissection and metacercarial aspiration for their transfer strongly reduce the cost price for metacercarial production of the trematodes Fasciola hepatica and Paramphistomum daubneyi.

Type
Research Papers
Information
Journal of Helminthology , Volume 88 , Issue 1 , March 2014 , pp. 105 - 111
Copyright
Copyright © Cambridge University Press 2012 

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References

Abrous, M., Roumieux, L., Dreyfuss, G., Rondelaud, D. & Mage, C. (1998) Proposition d'une technique simple pour la production métacercarienne de Fasciola hepatica Linné à partir du mollusque Lymnaea truncatula Müller. Revue de Médecine Vétérinaire de Toulouse 149, 943–948.Google Scholar
Barthe, D., Rondelaud, D., Faucher, Y. & Vago, C. (1984) Infection virale chez le Mollusque Pulmoné Lymnaea truncatula Müller. Comptes-Rendus de l'Académie des Sciences de Paris, D 298, 513–515.Google Scholar
Belfaiza, M., Abrous, M., Rondelaud, D., Moncef, M. & Dreyfuss, G. (2004) The use of Tetraphyll® as food for snails increases the intensity of cercarial shedding in Galba truncatula infected with Fasciola hepatica. Parasitology Research 94, 86–90.Google Scholar
Boray, J.C. (1969) Experimental fascioliasis in Australia. Advances in Parasitology 7, 95–210.Google Scholar
Bruce, J.J. & Liang, Y.S. (1992) Cultivation of schistosomes and snails for researchers in the United States of America and other countries. Journal of Medical and Applied Malacology 4, 13–30.Google Scholar
Dreyfuss, G., Abrous, M., Vignoles, P. & Rondelaud, D. (2004) Fasciola hepatica and Paramphistomum daubneyi: vertical distribution of metacercariae on plants under natural conditions. Parasitology Research 94, 70–73.Google Scholar
Dreyfuss, G., Vignoles, P. & Rondelaud, D. (2007) Fasciola hepatica: the infectivity of cattle-origin miracidia had increased over the past years in central France. Parasitology Research 101, 1157–1160.CrossRefGoogle ScholarPubMed
Fereira Filipa, M., Delgado, M.L., Seixa Lopes, A.M. & Sampaio Silva, M.L. (2000) Optimum conditions for growth in liquid medium of Oscillatoria formosa Bory used as the principal food in laboratory culture of intermediate hosts for schistosomosis and fasciolosis. Parasite 7, 227–231.Google Scholar
Gayout, M. (2009) La production des métacercaires de Fasciola hepatica. Saint Yrieix la Perche, Agricultural Secondary School.Google Scholar
Kendall, S.B. (1953) The life-history of Limnaea truncatula under laboratory conditions. Journal of Helminthology 27, 17–28.CrossRefGoogle Scholar
Ollerenshaw, C.B. (1971) Some observations on the epidemiology of fascioliasis in relation to the timing of molluscicide applications in the control of the disease. The Veterinary Record 88, 152–164.Google Scholar
Osborn, G.D., Gron, N. & Simmons, D. (1982) Maintenance and infection of the mud snail Lymnaea truncatula for Fasciola hepatica studies. Journal of the Institute of Animal Technicians 33, 1–5.Google Scholar
Pécheur, M. (1974) Lutte stratégique contre la distomatose. Comptes-Rendus de Recherches, Travaux du Centre de Recherches sur les Maladies Parasitaires des Animaux Domestiques 38, 85–150.Google Scholar
Reynal, J.L. (2001) Enquête épidémiologique sur les traitements appliqués contre la fasciolose et la paramphistomose bovine dans le Sud-ouest de la Corrèze. Pharm.D. thesis, Limoges.Google Scholar
Rondelaud, D. (1993) Variabilité interpopulationelle de l'infestation fasciolienne chez le mollusque Lymnaea truncatula Müller. Influence du contact préalable de la population avec le parasite. Bulletin de la Société Zoologique de France 118, 185–193.Google Scholar
Rondelaud, D., Abrous, M. & Dreyfuss, G. (2002) The influence of different food sources on cercarial production in Lymnaea truncatula experimentally infected with Digenea. Veterinary Research 33, 95–100.Google Scholar
Rondelaud, D., Denève, C., Belfaiza, M., Mekroud, A., Abrous, M., Moncef, M. & Dreyfuss, G. (2004) Variability in the prevalences of infections and cercarial production in Galba truncatula raised on a high quality diet. Parasitology Research 92, 242–245.Google Scholar
Rondelaud, D., Fousi, M., Vignoles, P., Moncef, M. & Dreyfuss, G. (2007) Optimization of metacercarial production for three digenean species by the use of Petri dishes for raising lettuce-fed Galba truncatula. Parasitology Research 100, 861–865.Google Scholar
Rondelaud, D., Vignoles, P. & Dreyfuss, G. (2009) La Limnée tronquée, un mollusque d'intérêt médical et vétérinaire. Limoges, PULIM.Google Scholar
Stat-Itcf, (1988) Manuel d'utilisation. Boigneville, Institut Technique des Céréales et des Fourrages, Service des Études Statistiques.Google Scholar
Taylor, E.L. & Mozley, A. (1948) A culture method for Lymnaea truncatula. Nature 161, 894.Google Scholar
Vareille-Morel, C., Dreyfuss, G. & Rondelaud, D. (1993) Premières données sur la dispersion et le devenir des métacercaires flottantes de Fasciola hepatica L. Bulletin de la Société Française de Parasitologie 11, 63–69.Google Scholar
Vignoles, P., Dreyfuss, G. & Rondelaud, D. (2002) Larval development of Fasciola hepatica in experimental infections: variations with populations of Lymnaea truncatula. Journal of Helminthology 76, 179–183.Google Scholar
Vignoles, P., Rondelaud, D. & Dreyfuss, G. (2008) Paramphistomum daubneyi: production dynamics and infectivity of metacercariae originating from snails dissected at regular intervals. Journal of Helminthology 82, 175–180.Google Scholar