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Association mapping and favourable allele exploration for plant architecture traits in upland cotton (Gossypium hirsutum L.) accessions

Published online by Cambridge University Press:  22 May 2015

C. Q. LI ,
N. J. AI ,
Y. J. ZHU ,
Y. Q. WANG ,
X. D. CHEN ,
F. LI ,
Q. Y. HU  and
Q. L. WANG
C. Q. LI
Affiliation:
Key Discipline Open Lab on Crop Molecular Breeding of Henan Institute of Higher Learning, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang 453003, Henan, China
N. J. AI
Affiliation:
Cotton Research Institute, Shihezi Agricultural Science and Technology Research Center, Shihezi 832000, China
Y. J. ZHU
Affiliation:
Zhumadian Academy of Agricultural Sciences, Zhumadian 463000, China
Y. Q. WANG
Affiliation:
Key Discipline Open Lab on Crop Molecular Breeding of Henan Institute of Higher Learning, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang 453003, Henan, China
X. D. CHEN
Affiliation:
Key Discipline Open Lab on Crop Molecular Breeding of Henan Institute of Higher Learning, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang 453003, Henan, China
F. LI
Affiliation:
Key Discipline Open Lab on Crop Molecular Breeding of Henan Institute of Higher Learning, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang 453003, Henan, China
Q. Y. HU
Affiliation:
Key Discipline Open Lab on Crop Molecular Breeding of Henan Institute of Higher Learning, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang 453003, Henan, China
Q. L. WANG*
Affiliation:
Key Discipline Open Lab on Crop Molecular Breeding of Henan Institute of Higher Learning, Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang 453003, Henan, China
*
*To whom all correspondence should be addressed. Email: cottonmol@aliyun.com
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Summary

Association mapping based on linkage disequilibrium (LD) is a promising tool to identify genes responsible for quantitative variations underlying complex traits. The present paper presents an association mapping panel consisting of 172 upland cotton (Gossypium hirsutum L.) accessions. The panel was phenotyped for five cotton plant architecture traits across multiple environments and genotyped using 386 simple sequence repeat (SSR) markers. Of these markers, 101 polymorphic SSR markers were used in the final analysis. There were abundant phenotypic variations within this germplasm panel and a total of 267 alleles ranging from two to seven per locus were identified in all collections. The threshold of LD decay was set to r2 = 0·1 and 0·2, and the genome-wide LD extended up to about 13–14 and 6–7 cM, respectively, providing the potential for association mapping of agronomically important traits in upland cotton. A total of 66 marker–trait associations were detected based on a mixed linear model, of which 35 were found in more than one environment. The favourable alleles from 35 marker loci can be used in marker-assisted selection of target traits. Both the synergistic alleles and the negative alleles for some traits, especially plant height and fruit branch angle, can be utilized in plant architecture breeding programmes according to specific breeding objectives.

Type
Crops and Soils Research Papers
Information
The Journal of Agricultural Science , Volume 154 , Issue 4 , May 2016 , pp. 567 - 583
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Copyright
Copyright © Cambridge University Press 2015 

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