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Nitrogen in the excreta of dairy cattle: changes during short-term storage

Published online by Cambridge University Press:  27 March 2009

D. C. Whitehead
Affiliation:
AFRC Institute of Grassland and Environmental Research, Hurley Research Station, Maidenhead, Berkshire SL6 SLR, UK
N. Raistrick
Affiliation:
AFRC Institute of Grassland and Environmental Research, Hurley Research Station, Maidenhead, Berkshire SL6 SLR, UK

Summary

The concentration of N in samples of urine from dairy cattle fed on grass herbage, or grass or maize silage, sometimes with additional concentrate feeds, ranged from 6·0 to 13·8 mg N/l with 67–91% of the total N being present as urea. The concentration of N in 11 samples of dung was 0·32–0·52% on a fresh weight basis (2·74–3·82% N in dry weight). About 18% of the dung N was contained in particulate material of > 0·2 mm diameter, c. 72% in fine particulate plus colloidal material, and c. 10% was soluble in the presence of A12(SO4)3.

When urine was stored for 3 weeks, the urea component was hydrolysed with the formation of ammonium. The rate at which hydrolysis occurred was greatly influenced by temperature. Hydrolysis of urea was complete within 2 days at 35 °C, within 7 days at 20 °C and within 21 days at 10 °C, but was only c. 90% complete after 21 days at 5 °C. The rate of hydrolysis of urinary urea-N at 20 °C was increased slightly by inoculation with slurry, dung or soil, and was also increased slightly by the greater aeration resulting from a continuous stream of bubbled air. No nitrification was detected, even in urine that was aerated for 6 weeks, probably because the process was inhibited under the conditions of high pH (9–10) and high concentrations of ammoniacal N.

When dung was stored for 3 weeks at 5 or 10 °C, there was little change in the amount of organic matter or in the form of N. However, at higher temperatures, some mineralization occurred and the amount of organic matter declined by 8% at 20 °C and by 17% at 35 °C. About 10% of the organic N was converted to ammonium during 3 weeks at 20 °C, and c. 18% at 35 °C.

With a slurry prepared from approximately equal amounts of urine, dung and water, more of the dung material was mineralized than with the dung stored alone: c. 15% of the organic matter was lost during 3 weeks at 5 °C and c. 34% at 35 °C. Despite this loss of organic matter, there was net immobilization of soluble N during the 3-week period by the solid fractions of the slurry, at all four temperatures.

Type
Crops and Soils
Copyright
Copyright © Cambridge University Press 1993

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References

REFERENCES

Besnard, C. (1980). Balance and evolution of nitrogen compounds during the treatment of slurry. In Effluents from Livestock (Ed. Gasser, J. K. R.), pp. 496506. London: Applied Science Publishers Ltd.Google Scholar
Bremner, J. M. & Mulvaney, R. L. (1978). Urease activity in soils. In Soil Enzymes (Ed. Burns, R. G.), pp. 149196. London: Academic Press.Google Scholar
Bristow, A. W., Whitehead, D. C. & Cockburn, J. E. (1992). Nitrogenous constituents in the urine of cattle, sheep and goats. Journal of the Science of Food and Agriculture 59, 387394.Google Scholar
Chalmers, A. G., Kershaw, C. D. & Leech, P. K. (1990). Fertilizer Use on Farm Crops, England and Wales, 1989. London: MAFF.Google Scholar
Dlaz-Flerros, F., Villar, M. C., Gil, F., Leirós, M. C.Carballas, M., Carballas, T. & Cabaneiro, A. (1987). Laboratory study of the availability of nutrients in physical fractions of cattle slurry. Journal of Agricultural Science, Cambridge 108, 353359.Google Scholar
Dewes, T., Schmitt, L., Valentin, U. & Ahrens, E. (1990). Nitrogen losses during the storage of liquid livestock manures. Biological Wastes 31, 241250.CrossRefGoogle Scholar
Follett, M. J. & Ratcliff, P. W. (1963). Determination of nitrite and nitrate in meat products. Journal of the Science of Food and Agriculture 14, 138144.Google Scholar
Gehrke, C. W., Kaiser, F. E. & Ussary, J. P. (1968). Automated spectrophotometric method for nitrogen in fertilizers. Journal of the Association of Official Agricultural Chemists 51, 200211.Google Scholar
Henriksen, A. & Selmer-Olsen, A. R. (1970). Automatic methods for determining nitrate and nitrite in water and soil extracts. Analyst 95, 514518.Google Scholar
Henzell, E. F. & Ross, P. J. (1973). The nitrogen cycle of pasture ecosystems. In Chemistry and Biochemistry of Herbage (eds Butler, G. W. & Bailey, R. W.), vol. 2, pp. 227246. London: Academic Press.Google Scholar
Lantinga, E. A., Keuning, J. A., Groenwold, J. & Deenen, P. J. A. G. (1987). Distribution of excreted nitrogen by grazing cattle and its effects on sward quality, herbage production and utilization. In Animal Manure on Grassland and Fodder Crops: Fertilizer or Waslel (Eds Meer, H. G. van der, Unwin, R. J., Dijk, T. A. Van & Ennik, G. C.), pp. 103117. Dordrecht: Martinus Nijhoff.Google Scholar
Loehr, R. C. (1977). Pollution Control for Agriculture. New York: Academic Press.Google Scholar
Marsh, W. H., Fingerhut, B. & Miller, M. (1965). Automated and manual direct methods for the determination of blood urea. Clinical Chemistry 11, 624627.Google Scholar
Ministry of Agriculture, Fisheries and Food (1976). Organic Manures. MAFF (ADAS) Bulletin no. 210. London: HMSO.Google Scholar
Royal Society (1983). The Nitrogen Cycle of the United Kingdom, a Study Group Report. London: The Royal Society.Google Scholar
Sherlock, R. R. & Goh, K. M. (1984). Dynamics of ammonia volatilization from simulated urine patches and aqueous urea applied to pasture. I Field experiments. Fertilizer Research 5, 181195.CrossRefGoogle Scholar
Thomas, R. J., Logan, K. A. B., Ironside, A. D. & Bolton, G. R. (1988). Transformations and fate of sheep urine-N applied to an upland U.K. pasture at different times during the growing season. Plant and Soil 107, 173181.CrossRefGoogle Scholar
Topps, J. H. & Elliott, R. C. (1966). Partition of nitrogen in the urine of African sheep given low-protein diets. Proceedings of the Nutrition Society 25, xixxx.Google Scholar
Warren, G. P. & Whitehead, D. C. (1988). Available soil nitrogen in relation to fractions of soil nitrogen and other soil properties. Plant and Soil 112, 155165.Google Scholar
Weatherburn, M. W. (1967). Phenol-hypochlorite reaction for determination of ammonia. Analytical Chemistry 39, 971974.Google Scholar
Whitehead, D. C., Bristow, A. W. & Pain, B. F. (1989). The influence of some cattle and pig slurries on the uptake of nitrogen by ryegrass in relation to fractionation of the slurry N. Plant and Soil 117, 111120.CrossRefGoogle Scholar